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CircPSMC3 inhibits cell proliferation and induces cell apoptosis in nasopharyngeal carcinoma by downregulating ROCK1

D-Q Guo1, F Liu, L Zhang

  • 1Department of Otolaryngology Head and Neck Surgery, People's Liberation Army Hospital 960 Ziboyuan District, Zibo, China. 1811592516@qq.com.

European Review for Medical and Pharmacological Sciences
|February 26, 2020
PubMed
Abstract

Insights

Circular RNA circPSMC3 suppresses nasopharyngeal carcinoma (NPC) cell growth and promotes apoptosis by downregulating ROCK1. Low circPSMC3 expression correlates with poor prognosis in NPC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in various cancers.
  • The specific function of circPSMC3 in nasopharyngeal carcinoma (NPC) has not been elucidated.
  • Understanding circRNA mechanisms is crucial for developing novel cancer therapies.

Purpose of the Study:

  • To investigate the role of circPSMC3 in the proliferation and apoptosis of NPC.
  • To explore the underlying molecular mechanisms of circPSMC3 in NPC.
  • To determine the prognostic value of circPSMC3 in NPC patients.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to assess circPSMC3 expression levels in NPC tissues and cell lines.
  • In vitro assays (CCK-8, colony formation, flow cytometry) to evaluate cell proliferation and apoptosis after circPSMC3 manipulation.
  • Western blot analysis to identify circPSMC3 target proteins.
  • In vivo studies in nude mice to assess the effect of circPSMC3 on tumor formation.

Main Results:

  • CircPSMC3 expression was significantly downregulated in NPC tissues and cell lines compared to normal controls.
  • Low circPSMC3 expression was associated with poorer prognosis in NPC patients.
  • Overexpression of circPSMC3 inhibited NPC cell proliferation, promoted apoptosis in vitro, and suppressed tumor formation in vivo.
  • CircPSMC3 overexpression led to a marked decrease in ROCK1 expression.

Conclusions:

  • CircPSMC3 acts as a tumor suppressor in NPC.
  • CircPSMC3 inhibits NPC cell growth and induces apoptosis by downregulating ROCK1.
  • CircPSMC3 holds potential as a prognostic biomarker and therapeutic target for NPC.

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