Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Dark Triad and Person Perception01:29

Dark Triad and Person Perception

277
Person perception is influenced by both external behaviors and the observer’s internal characteristics, including personality traits. Individuals with dark personality traits, comprising psychopathy, Machiavellianism, and narcissism — collectively known as the dark triad – exhibit manipulative and exploitative tendencies in social contexts. These traits affect how they perceive others and how they are perceived.The Role of Dark Personality Traits in Person PerceptionBlack et...
277
Visual System01:26

Visual System

1.7K
Light enters the eye through the cornea, a transparent, dome-shaped surface covering the surface of the eyeball that helps to direct and focus incoming light. This light is then channeled toward the pupil, an adjustable opening whose size is controlled by the iris. The iris, a pigmented muscle, regulates the amount of light entering the eye by contracting or dilating the pupil, thereby ensuring optimal light levels for clear vision.
Once through the pupil, the light passes through the lens, a...
1.7K
Visual Agnosia01:12

Visual Agnosia

1000
Visual agnosia is a condition characterized by the inability to recognize visually presented objects despite having normal vision. For instance, a person with visual agnosia can describe the shape and color of an object but cannot identify or name it. This impairment does not affect their visual field, acuity, color vision, brightness discrimination, language, or memory. An example of this condition in a social setting is someone at a dinner party asking for "that silver thing with a round...
1000
Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

8.8K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
8.8K
Euchromatin01:01

Euchromatin

8.8K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
8.8K
Interference and Diffraction02:18

Interference and Diffraction

51.8K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
51.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early Surge in Brain GFAP Distribution in Female Hamsters With Mild Peripheral COVID-19.

Journal of neurochemistry·2026
Same author

Vagus nerve stimulation as an anti-inflammatory therapy for maternal immune activation-induced alterations in offspring microglia and neurodevelopment.

Frontiers in neuroscience·2026
Same author

Neuronal lipid droplets play a conserved and sex-biased role in maintaining whole-body energy homeostasis.

Nature metabolism·2026
Same author

Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice.

Brain, behavior, & immunity - health·2026
Same author

Glioma-induced DNMT3A reduction in microglia promotes an anti-tumoral phenotype.

Cell death and differentiation·2026
Same author

Runx1 transcription factor modulates opioid analgesia and withdrawal in humans and rodents.

Neuron·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jan 21, 2026

Generation of Human Microglia to Combine Them with Retinal Organoids for Improved Disease Modeling
06:24

Generation of Human Microglia to Combine Them with Retinal Organoids for Improved Disease Modeling

Published on: July 26, 2024

1.9K

Visualizing Dark Microglia.

Marie-Kim St-Pierre1,2, Maude Bordeleau1,3, Marie-Ève Tremblay4,5

  • 1Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, Québec, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|August 9, 2019
PubMed
Summary
This summary is machine-generated.

Dark microglia, a recently identified cell type, are abundant in neurological diseases and aging. This protocol details transmission electron microscopy methods for their identification and ultrastructural analysis, enabling new research avenues.

Keywords:
BrainDark microgliaMouseTransmission electron microscopyUltrastructural characterization

More Related Videos

Author Spotlight: Using Zebrafish to Explore Microglia Migration During Brain Development
07:45

Author Spotlight: Using Zebrafish to Explore Microglia Migration During Brain Development

Published on: May 17, 2024

2.0K
In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus
07:03

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

Published on: July 6, 2022

4.9K

Related Experiment Videos

Last Updated: Jan 21, 2026

Generation of Human Microglia to Combine Them with Retinal Organoids for Improved Disease Modeling
06:24

Generation of Human Microglia to Combine Them with Retinal Organoids for Improved Disease Modeling

Published on: July 26, 2024

1.9K
Author Spotlight: Using Zebrafish to Explore Microglia Migration During Brain Development
07:45

Author Spotlight: Using Zebrafish to Explore Microglia Migration During Brain Development

Published on: May 17, 2024

2.0K
In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus
07:03

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

Published on: July 6, 2022

4.9K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Pathology

Background:

  • Dark microglia represent a distinct cellular phenotype observed in non-homeostatic conditions such as Alzheimer's disease, aging, and chronic stress.
  • Current limitations in studying dark microglia stem from the absence of a specific protein marker, precluding conventional cellular biology techniques.
  • These cells are identifiable by unique ultrastructural features observable via electron microscopy.

Purpose of the Study:

  • To provide a detailed protocol for the preparation of high-quality brain tissues for transmission electron microscopy (TEM).
  • To outline methods for the imaging and identification of dark microglia using TEM.
  • To describe the ultrastructural analysis of parameters within dark microglia.

Main Methods:

  • Tissue preparation for high-resolution transmission electron microscopy.
  • Nanoscale resolution imaging techniques for cellular identification.
  • Ultrastructural analysis of cellular morphology and parameters.

Main Results:

  • Successful preparation of brain tissues suitable for high-quality TEM imaging.
  • Demonstration of dark microglia identification based on unique ultrastructural features.
  • Establishment of methods for quantitative ultrastructural analysis of dark microglia.

Conclusions:

  • This protocol enables the study of dark microglia, a phenotype implicated in various neurological conditions.
  • The described TEM methods overcome the lack of specific markers, facilitating research into dark microglia biology.
  • This work provides a foundation for understanding the role of dark microglia in health and disease.