An Orbitide from Ratibida columnifera Seed Containing 16 Amino Acid Residues
Mark F Fisher1, Colton D Payne2, K Johan Rosengren2
1School of Molecular Sciences , The University of Western Australia , 35 Stirling Highway , Crawley , WA 6009 , Australia.
Abstract:
Cyclic peptides are abundant in plants and have attracted interest due to their bioactivity and potential as drug scaffolds. Orbitides are head-to-tail cyclic peptides that are ribosomally synthesized, post-translationally modified, and lack disulfide bonds. All known orbitides contain 5-12 amino acid residues. Here we describe PLP-53, a novel orbitide from the seed of Ratibida columnifera. PLP-53 consists of 16 amino acids, four residues larger than any known orbitide. NMR structural studies showed that, compared to previously characterized orbitides, PLP-53 is more flexible and, under the studied conditions, did not adopt a single ordered conformation based on analysis of NOEs and chemical shifts.
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