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Related Experiment Video

Updated: Jan 21, 2026

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Published on: February 12, 2022

Unlimited Genetic Switches for Cell-Type-Specific Manipulation.

Jorge Garcia-Marques¹, Ching-Po Yang¹, Isabel Espinosa-Medina¹

¹Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

|August 10, 2019

Summary

Researchers developed CaSSA, a novel CRISPR/Cas9-based platform enabling precise genetic targeting of specific cell types. This tool facilitates complex genetic manipulations by creating unlimited, customizable genetic switches for diverse applications.

Keywords:

CRISPR/Cas9 CaSSA Drosophila cell-type-specific gene trap genetic access genetic intersection recombinase single-strand annealing zebrafish

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Last Updated: Jan 21, 2026

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

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Published on: February 12, 2022

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

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Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging

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Published on: September 17, 2009

Area of Science:

Molecular Biology
Genetics
Bioengineering

Background:

Precise genetic manipulation of distinct cell types is essential for biological research and gene therapy.
Transcriptomics reveals cell types are defined by unique genetic markers, but tools for multi-marker targeting are lacking.
Current methods limit access to many cell types for genetic intervention.

Purpose of the Study:

To introduce CaSSA, a new platform for creating versatile genetic switches.
To enable multiplex, cell-type-specific genetic manipulations using CRISPR/Cas9 and single-strand annealing.
To overcome limitations in targeting cells based on multiple genetic markers.

Main Methods:

CaSSA platform utilizes CRISPR/Cas9 (Ca) and single-strand annealing (SSA) DNA repair.
Engineering of independent genetic switches, each responsive to a specific guide RNA (gRNA).
Combinatorial targeting achieved by expressing multiple gRNAs in specific patterns.

Main Results:

CaSSA provides a method for creating an unlimited number of genetic switches.
The platform enables multiplex cell-type-specific genetic targeting.
Demonstrates potential for complex genetic interventions in various organisms.

Conclusions:

CaSSA offers a powerful new tool for unprecedented genetic access to cell types.
Facilitates cell-type-specific gene therapy and biological function studies.
Expands possibilities for genetic engineering across diverse biological systems.