Related Experiment Video

Updated: Jan 21, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.8K

First Whole-Genome Sequence of a Highly Resistant Klebsiella pneumoniae Sequence Type 14 Strain Isolated from Sudan

Sofia B Mohamed1, Sumaya Kambal2, Abdalla Munir2

  • 1Bioinformatics and Biostatistics Department, National University Biomedical Research Institute, National University, Khartoum, Sudan sofiabashir2002@gmail.com.

Microbiology Resource Announcements
|August 10, 2019
PubMed

Insights

This study details the genome of a highly drug-resistant Klebsiella pneumoniae ST14 strain from Sudan. Understanding this pathogen is crucial for combating hospital-acquired infections.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Klebsiella pneumoniae is a major cause of hospital-acquired infections.
  • It is a leading pathogen in nosocomial outbreaks globally.
  • Antimicrobial resistance in K. pneumoniae poses a significant public health threat.

Purpose of the Study:

  • To sequence and characterize a highly resistant Klebsiella pneumoniae strain.
  • To provide genomic data for a strain belonging to sequence type 14 (ST14).
  • To contribute to understanding the genetic basis of resistance in K. pneumoniae.

Main Methods:

  • Whole-genome sequencing of the K. pneumoniae ST14 isolate.
  • Bioinformatic analysis of the genomic data.
  • Identification of antimicrobial resistance genes and genomic features.

Main Results:

  • The complete genomic sequence of the K. pneumoniae ST14 strain was obtained.
  • The genome revealed the presence of multiple antimicrobial resistance genes.
  • Specific genomic features associated with virulence and adaptation were identified.

Conclusions:

  • The genomic data provides insights into the genetic makeup of a highly resistant K. pneumoniae ST14 strain.
  • This information can aid in the development of targeted interventions against K. pneumoniae infections.
  • Further research is needed to explore the implications of these findings for clinical management and public health strategies.

More Related Videos

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.4K
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

14.2K

Related Concept Videos

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

4.0K
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
773.4K
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.7K
Per-Unit Sequence Models01:26

Per-Unit Sequence Models

An ideal Y-Y transformer, grounded through neutral impedances, displays per-unit sequence networks akin to those of a single-phase ideal transformer when subjected to balanced positive- or negative-sequence currents. These currents do not produce neutral currents, and their associated voltage drops.
Zero-sequence currents, which are identical in magnitude and phase, generate a neutral current, resulting in voltage drops across the neutral impedance and the low-voltage winding. If the...
432