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Related Concept Videos

Mutation, Gene Flow, and Genetic Drift01:09

Mutation, Gene Flow, and Genetic Drift

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In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
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Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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Viral Mutations00:36

Viral Mutations

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A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
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Mutations01:39

Mutations

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Overview
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Mutations01:35

Mutations

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Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
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Isothermal Processes01:21

Isothermal Processes

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A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes.
An ideal gas can also undergo isothermal expansion or compression.
For example, consider 1 mole of an ideal gas inside an isolated cylinder at initial volume V...
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Related Experiment Video

Updated: Jan 21, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
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Loop Mediated Isothermal Amplification: A Promising Tool for Screening Genetic Mutations.

Arjuna Srividya1, Biswajit Maiti2, Anirban Chakraborty1

  • 1Division of Molecular Genetics and Cancer, Nitte University Centre for Science Education and Research, Paneer Campus, Deralakatte, Mangaluru, 575018, India.

Molecular Diagnosis & Therapy
|August 10, 2019
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Summary

Loop-mediated isothermal amplification (LAMP) offers a rapid, sensitive, and cost-effective method for genetic diagnosis. This review explores LAMP assay applications for screening single-nucleotide polymorphisms and gene translocations in cancer.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Mutation screening is crucial for clinical diagnosis and therapeutic strategies.
  • Nucleic acid-based techniques are highly accurate for genetic diagnosis.
  • Loop-mediated isothermal amplification (LAMP) is a rapid, sensitive, and cost-effective nucleic acid amplification technique.

Purpose of the Study:

  • To review the application of loop-mediated isothermal amplification (LAMP) and its variants in mutation screening.
  • To highlight the potential of LAMP assays for detecting single-nucleotide polymorphisms (SNPs) and gene translocations in cancer.

Main Methods:

  • Review of existing literature on loop-mediated isothermal amplification (LAMP) assays.
  • Analysis of LAMP assay variants developed for mutation detection.
  • Discussion of the principles and advantages of LAMP for genetic analysis.

Main Results:

  • LAMP assays demonstrate significant advantages in rapidity, efficiency, sensitivity, and cost.
  • While widely used in pathogen detection and crop development, LAMP's application in mutation screening has been limited.
  • Variants of LAMP have been developed for screening single-nucleotide polymorphisms and gene translocations.

Conclusions:

  • The LAMP assay presents a promising tool for mutation screening, particularly for cancer-related genetic alterations.
  • Further development and application of LAMP variants can enhance genetic diagnosis and personalized medicine.
  • LAMP's isothermal nature and high efficiency make it suitable for diverse diagnostic applications.