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Related Experiment Videos

Restricted Ig variable region gene expression among Ly-1+ B cell lymphomas.

C A Pennell1, L W Arnold, G Haughton

  • 1Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.

Journal of Immunology (Baltimore, Md. : 1950)
|October 15, 1988
PubMed
Summary

Ly-1+ B cell lymphomas share common B cell receptor structures due to using the same V genes. Antigen selection likely drives this restricted V gene usage in lymphomas.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • Ly-1+ B cell lymphomas (CH series) exhibit shared surface immunoglobulin (Ig) features.
  • These shared features include antigen specificity and idiotype.

Purpose of the Study:

  • To investigate the genetic basis for structural similarities in Ly-1+ B cell lymphomas.
  • To determine the V gene usage in CH lymphomas and its relation to shared idiotype and specificity.

Main Methods:

  • Sequencing of VH and VL region genes from 10 CH lymphomas.
  • Southern blot analysis of VH and V kappa gene rearrangements.
  • Comparison of gene sequences and rearrangements among CH lymphomas.

Main Results:

Related Experiment Videos

  • Identified a limited repertoire of five VH and seven VL genes used in CH lymphomas.
  • Found that these V genes are largely unmutated.
  • Demonstrated that lymphomas expressing identical VH genes share idiotope(s).
  • Observed no preferential usage of specific VH families or VL groups.

    • Conclusions:

    • Shared idiotype and specificity in CH lymphomas are primarily due to the use of the same V genes.
    • The restriction in V gene expression is not explained by specific V gene family or group preference.
    • Hypothesize that antigen selection is the driving force behind restricted V gene usage in these lymphomas.