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Related Experiment Video

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All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

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Resolving Single-Nanoconstruct Dynamics during Targeting and Nontargeting Live-Cell Membrane Interactions.

Debanjan Bhowmik, Kayla S B Culver, Tingting Liu

|August 10, 2019

Summary

This summary is machine-generated.

Targeted nanoparticles show distinct dynamics on cell surfaces, revealing insights into nanoparticle-cell interactions. This research aids in designing better nanoparticle delivery vehicles by analyzing their movement and interactions.

Keywords:

differential interference contrast microscopy membrane−receptor interactions protein corona single-particle dynamics targeting and nontargeting nanoconstructs

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All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

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Published on: January 19, 2018

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Area of Science:

Nanotechnology
Biophysics
Materials Science

Background:

Understanding nanoparticle (NP)-cell interactions is crucial for developing effective drug delivery systems.
The protein corona formed on NPs can influence their biological behavior and targeting efficiency.
Characterizing NP dynamics provides insights into their interactions with cell membranes.

Purpose of the Study:

To investigate the translational and rotational dynamics of functionalized gold nanostar (AuNS) nanoconstructs interacting with cells.
To compare the membrane dynamics of targeted AuNS with nontargeting AuNS in serum-containing medium.
To determine if targeted interactions are maintained despite protein adsorption.

Main Methods:

Utilized single nanoparticle tracking to probe the dynamics of functionalized Au nanostar (AuNS) nanoconstructs.
Studied AuNS interacting with cell membranes expressing human epidermal growth factor receptor 2 (HER-2).
Analyzed translational and rotational dynamics in serum-containing medium.

Main Results:

AuNS with targeting ligands exhibited a larger dynamical footprint and faster rotational speed on HER-2-expressing cells compared to nontargeting AuNS.
Targeting and nontargeting nanoconstructs showed distinct membrane dynamics.
These distinct dynamics persisted despite similar protein adsorption profiles, indicating preserved targeted interactions.

Conclusions:

Single nanoparticle dynamics are sensitive to nanoconstruct properties and cell interactions.
Targeted nanoparticle-cell interactions are preserved even in the presence of a protein corona.
Analyzing NP dynamics can guide the design of improved nanoparticle delivery vehicles by optimizing size, shape, and surface chemistry.