Related Experiment Video
Updated: Jan 21, 2026

Protocols for Quantifying Transferable Pesticide Residues in Turfgrass Systems
Published on: March 15, 2017
Click Reaction-Mediated T2 Immunosensor for Ultrasensitive Detection of Pesticide Residues via Brush-like
Yongzhen Dong1,2, Wenshu Zheng3, Da Chen4
1College of Food Science and Technology , Huazhong Agricultural University , Wuhan , Hubei 430070 , China.
Abstract:
We develop an ultrasensitive T2-mediated immunosensor based on the coordination chemistry and Cu(I)-catalyzed 1,3-dipolar cycloaddition of azide andalkyne (CuAAC) and apply it for the detection of pesticide residues. We functionalize polyglutamic acid (PGA) on polystyrene to form a brush-like nanostructure that has a large loading capacity of Cu(II) through the coordination chemistry between PGA and Cu(II). Such a brush-like nanostructure could be used to chelate Cu(II) to modulate the CuAAC between azide-functionalized 1000 nm polystyrene (PS1000) and alkyne-functionalized 30 nm magnetic nanoparticles (MNP30), and the MNP30-PS1000 conjugate as a product of CuAAC can act as a magnetic probe in this T2-based immunosensor. This click chemistry and coordination chemistry-mediated immunosensor allows for an ultrasensitive detection for chlorpyrifos residue (0.022 ng/mL), a 58-fold enhancement compared with that of enzyme-linked immunosorbent assay (1.28 ng/mL), providing a promising platform for detection of trace small molecules.
Related Concept Videos
Coordination Number and Geometry
Coordination Compounds and Nomenclature
Residual Stresses
Residual Plots
When the residual values are plotted against the variable x, it is called a residual...
Lattice Centering and Coordination Number
Types of Unit Cells
Imagine taking a large number of identical...
Equations of Motion: Rectangular Coordinates and Cylindrical Coordinates
When a particle moves relative to an inertial frame, the equations of motion can be expressed using rectangular components. If the motion is confined to the x-y plane, the equations having the x and y coordinates only can be used to simplify the mathematical representation.
However, when particles...

