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High Accurate Micro-Orifice Resistance Assay with Programmable Aggregation-Dispersion Switching Enabled by an
Feng Hong1,2, Liangqiong Ren1,3, Wenshu Zheng4
1State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
Analytical Chemistry
|April 20, 2026
Summary
This study introduces a novel biosensor for rapid and sensitive pathogenic bacteria detection. The micro-orifice resistance biosensor uses a molecular circuit to convert bacterial DNA into a quantifiable electrical signal, enabling quick pathogen analysis.
Area of Science:
- Biotechnology
- Biosensors
- Molecular Diagnostics
Background:
- Pathogenic bacterial detection faces challenges in signal amplification and stability.
- Existing methods often lack sensitivity or are susceptible to environmental interference.
Purpose of the Study:
- To develop a rapid and sensitive biosensor for pathogenic bacteria detection.
- To overcome limitations of current detection strategies using a novel molecular circuit approach.
Main Methods:
- A micro-orifice resistance biosensor was designed utilizing a molecular circuit for aggregation-dispersion switching.
- A hairpin-free bridge-hybridization chain reaction (nHCR) was employed for signal amplification.
- The biosensor converts bacterial DNA recognition into quantifiable electrical resistance pulse signatures.
Main Results:
- The biosensor achieved a limit of detection of 27 CFU/mL for *Listeria monocytogenes* without amplification.
- The molecular circuit demonstrated robust signal transduction, converting molecular events into electrical signals.
- Performance was comparable to quantitative PCR in real sample analysis.
Conclusions:
- The developed biosensor offers a robust and scalable strategy for rapid pathogen analysis.
- Molecular circuit-programmed dispersion switching provides a novel mechanism for sensitive bacterial detection.
- This approach enhances signal gain and stability for reliable diagnostics.
