Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

3.0K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
3.0K
Magical Thinking01:29

Magical Thinking

196
Magical thinking encompasses the belief in assumptions that defy logical reasoning yet appear intuitively convincing. It is a common psychological phenomenon that persists across various cultural and individual contexts. While these assumptions contradict empirical evidence and scientific laws, they often serve meaningful psychological roles in promoting emotional resilience and a sense of control, especially under stress or uncertainty.Thought-Action Fusion and the Law of SimilarityA key...
196
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses01:25

Determination of Multiple Dosing Parameters: Loading and Maintenance Doses

228
A loading dose is an essential pharmacological strategy to rapidly achieve the target plasma drug concentration necessary for an immediate therapeutic effect. This approach is especially critical for drugs characterized by slow absorption or extended half-lives, where delaying therapeutic plasma levels could compromise treatment outcomes. By administering a loading dose, clinicians ensure a prompt onset of drug action, even for agents with complex pharmacokinetic profiles.Achieving steady-state...
228
Metallic Solids02:37

Metallic Solids

20.5K
Metallic solids such as crystals of copper, aluminum, and iron are formed by metal atoms. The structure of metallic crystals is often described as a uniform distribution of atomic nuclei within a “sea” of delocalized electrons. The atoms within such a metallic solid are held together by a unique force known as metallic bonding that gives rise to many useful and varied bulk properties.
All metallic solids exhibit high thermal and electrical conductivity, metallic luster, and malleability....
20.5K
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations01:15

Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations

239
Gentamicin, an aminoglycoside antibiotic, is commonly administered via intermittent intravenous infusion to treat severe infections. An intermittent one-hour infusion of gentamicin, administered at eight-hour intervals, allows for precise control of plasma drug concentrations, minimizing toxicity while ensuring therapeutic efficacy. Pharmacokinetic principles govern the dynamics of plasma concentrations and can be mathematically described using specific equations.The plasma drug concentration...
239
Structures of Solids02:22

Structures of Solids

17.5K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
17.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanistic Insights Into Metal Binding in the Aggregation Pathway of an Immunoglobulin Light Chain Protein.

Chembiochem : a European journal of chemical biology·2026
Same author

Serial effects in choice and confidence modulate each other: Evidence from 26 experiments.

Cognition·2026
Same author

Accelerating Reaction Discovery through AI-HTE Integration: Nitrene-Mediated C-O Coupling as a Validated Case Study.

JACS Au·2026
Same author

A novel surgical T staging system for recurrent nasopharyngeal carcinoma: advancing prognostic accuracy and clinical applicability.

Journal of neurosurgery·2026
Same author

Metabolic reprogramming of glutamine is associated with M2 macrophage polarization in allergic rhinitis.

Frontiers in immunology·2026
Same author

Ruxolitinib Penetrates Blood Brain Barrier and Reduces the Cytokine Storm in Patients With Haemophagocytic Lymphohistiocytosis.

Journal of cellular and molecular medicine·2026

Related Experiment Video

Updated: Jan 21, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

6.0K

Determination of methyl order parameters using solid state NMR under off magic angle spinning.

Kai Xue1,2, Salvatore Mamone3, Benita Koch1,2

  • 1Helmholtz-Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt, Ingolstädter Landstr. 1, 85764, Neuherberg, Germany.

Journal of Biomolecular NMR
|August 14, 2019
PubMed
Summary

This study quantifies methyl order parameters in proteins using a novel spin-echo method. The technique accurately measures molecular dynamics in biological solids, crucial for understanding protein function.

Keywords:
Microcrystalline proteinsOff magic angle spinningPerdeuterationSolid-state NMRSpin Echo

More Related Videos

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
07:33

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

Published on: April 15, 2010

12.9K
Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
11:44

Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes

Published on: November 12, 2016

18.6K

Related Experiment Videos

Last Updated: Jan 21, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

6.0K
Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
07:33

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

Published on: April 15, 2010

12.9K
Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
11:44

Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes

Published on: November 12, 2016

18.6K

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy
  • Biophysical chemistry
  • Structural biology

Background:

  • Quantifying dipolar couplings in biological solids is key to understanding molecular dynamics.
  • Magic Angle Spinning (MAS) with radio frequency pulses is typically used to obtain order parameters.
  • A recent method allows accurate estimation of amide backbone order parameters via a mis-calibrated rotor spinning angle in spin-echo experiments.

Purpose of the Study:

  • To apply the novel spin-echo method for determining methyl order parameters.
  • To investigate the dynamics of methyl-containing side chains in proteins.

Main Methods:

  • Utilized a spin-echo experiment with a slightly mis-calibrated rotor spinning angle.
  • Applied the method to a deuterated sample of the SH3 domain of chicken α-spectrin.
  • Focused on selectively protonated valine and leucine methyl groups.

Main Results:

  • Successfully determined methyl order parameters using the developed technique.
  • Demonstrated the applicability of the method to methyl groups in complex biological systems.
  • Provided insights into the dynamic behavior of methyl-containing side chains.

Conclusions:

  • The spin-echo method with a mis-calibrated rotor angle is effective for quantifying methyl order parameters.
  • This approach offers a valuable tool for studying protein dynamics in biological solids.
  • The findings contribute to a deeper understanding of molecular motion in proteins.