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Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing

Ang Hu1, Yahai Lu2, Marcela Hernández García3,4

  • 1College of Resources and Environment, Hunan Agricultural University, Changsha, China. anghu@hunau.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|August 14, 2019
PubMed
Summary

This study combines RNA stable isotope probing (SIP) with metatranscriptomics to identify active microbial populations and their gene expression. The protocol targets methanotrophs in rice field soil for enhanced microbial community analysis.

Keywords:
13C-mRNAHigh-throughput sequencingMetatranscriptomeSoil microbiomeStable isotope probing

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