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Updated: Jan 21, 2026

Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection
Published on: August 27, 2021
Complete genome sequence and characterization of a new iflavirus from the small brown planthopper (Laodelphax
Nan Wu1, Peipei Zhang2, Wenwen Liu1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Abstract:
A novel iflavirus, tentatively named laodelphax striatellus iflavirus 1 (LsIV1), was identified in Laodelphax striatellus by total RNA-sequencing, and its genome sequence was confirmed by Sanger sequencing. The complete genome consisted of 10,831 nucleotides with a polyA tail and included one open reading frame, encoding a 361.7-kD polyprotein. Conserved motifs for structural proteins, helicase, protease, and RNA-dependent RNA polymerase were identified by aligning the deduced amino acid sequence of LsIV1 with several other iflaviruses. The genome has the highest identity with another planthopper iflavirus, nilaparvata lugens honeydew virus-3 (39.7%), under the species demarcation threshold (90%). Results of the identities and phylogenetic analysis based on the deduced amino acid sequences of the complete polyprotein and helicase of LsIV1 and other iflaviruses, indicated it is a new species belonging to the family Iflaviridae. Furthermore, we did not observe any differences of biological characterizations like development and reproduction between viruliferous and virus-free SBPH. Meanwhile, we found that female could transmit LsIV1 with higher transmission efficiency.
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