Related Experiment Video

Updated: Jan 21, 2026

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
08:42

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Published on: July 19, 2019

10.7K

miR-625 reverses multidrug resistance in gastric cancer cells by directly targeting ALDH1A1

Xufei Gong1, Baoli Xu1, Li Zi1

  • 1Department of General Surgery, Linyi People's Hospital, Linyi, Shandong 276002, People's Republic of China.

Cancer Management and Research
|August 15, 2019
PubMed

Insights

MicroRNA-625 (miR-625) reverses multidrug resistance (MDR) in gastric cancer by targeting aldehyde dehydrogenase 1A1 (ALDH1A1). This finding offers potential for overcoming MDR in gastric cancer chemotherapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of multidrug resistance (MDR) in gastric cancer.
  • Gastric cancer MDR is a primary reason for chemotherapy failure.
  • miR-625 is downregulated in gastric cancer and linked to metastasis.

Purpose of the Study:

  • To investigate the role of miR-625 in regulating MDR in gastric cancer.
  • To determine if miR-625 can overcome MDR in gastric cancer cells.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) to measure miR-625 levels.
  • MTT assay to assess chemosensitivity.
  • Western blot and luciferase reporter assays to identify miR-625 targets.

Main Results:

  • miR-625 was significantly downregulated in MDR gastric cancer cells.
  • miR-625 increased gastric cancer cell sensitivity to chemotherapeutic agents and promoted apoptosis.
  • miR-625 directly targets aldehyde dehydrogenase 1A1 (ALDH1A1); restoring ALDH1A1 reversed miR-625's effects on MDR.

Conclusions:

  • miR-625 reverses MDR in gastric cancer by targeting ALDH1A1.
  • miR-625 is a novel regulator of MDR in gastric cancer.
  • miR-625 holds potential for overcoming MDR in gastric cancer chemotherapy.
Keywords:
ALDH1A1apoptosisgastric cancermiR-625multidrug resistance

More Related Videos

In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging09:29

In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging

Published on: April 28, 2017

9.9K
Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue06:36

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Published on: May 1, 2019

7.4K

Related Concept Videos

Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.7K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K
Gastric Motility01:16

Gastric Motility

Gastric motility is the coordinated contraction and relaxation of stomach muscles that convert ingested food into chyme, a semi-liquid substance ready for further digestion in the intestines. The process begins with the vagus nerve inducing the relaxation of the smooth muscles in the fundus and body of the stomach, allowing these regions to expand and accommodate up to approximately 1.5 liters of food and liquid.
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
2.9K
Gastric Emptying01:16

Gastric Emptying

Gastric emptying occurs when the stomach gradually releases chyme into the duodenum. When the stomach is distended, it triggers the release of gastrin, a hormone that promotes gastric acid secretion to aid in digestion. Additionally, stomach distension contributes to peristaltic waves that propel gastric contents toward the pyloric region. The gastroenteric reflex, on the other hand, primarily stimulates peristalsis in the intestines, facilitating the movement of contents further along the...
3.4K
Gastric Phase of Digestion01:26

Gastric Phase of Digestion

The gastric phase of digestion begins as soon as food enters the stomach. The incoming food bolus triggers neural and hormonal mechanisms, which last approximately 3 to 4 hours. During this phase, the stomach undergoes significant changes to prepare the food for further digestion and absorption.
When food enters the stomach, it stretches the stomach walls and activates stretch receptors. This triggers local reflexes of the enteric nervous system, mediated through the myenteric plexus. These...
3.1K
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.4K