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Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
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The prognostic significance of SHP2 and its binding protein Hook1 in non-small cell lung cancer

Lingjuan He1, Yinyan Li1, Xin Huang1

  • 1Department of Pharmacy, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310000, Zhejiang, People's Republic of China.

Oncotargets and Therapy
|August 16, 2019
PubMed
Abstract

Insights

High SHP2 protein expression correlates with poor prognosis in non-small cell lung cancer (NSCLC). This study analyzed SHP2 and Hook1 expression, finding SHP2 is an independent prognostic factor for overall survival in NSCLC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Previous research indicated Hook1 inhibits SHP2 phosphatase activity in epithelial-mesenchymal transition (EMT) regulation within lung cancer.
  • This study comprehensively analyzes SHP2 and Hook1 expression and their prognostic implications in non-small cell lung cancer (NSCLC).

Purpose of the Study:

  • To investigate the expression patterns of SHP2 and Hook1 in NSCLC.
  • To determine the relationship between SHP2 and Hook1 expression and patient prognosis in NSCLC.

Main Methods:

  • Immunohistochemistry and Western blot analysis were used to assess SHP2 and Hook1 expression in 121 NSCLC patients.
  • Overall survival rates were analyzed using Cox's hazard ratio multivariate analysis and the log-rank test.

Main Results:

  • SHP2 protein expression was observed in 58.4% of NSCLC tumor specimens.
  • A significant correlation was found between SHP2 and Hook1 expression. Hook1 was downregulated, and SHP2 showed a non-significant tendency to overexpress in NSCLC compared to normal lung tissue.
  • Low SHP2 expression was associated with significantly better overall survival (median 40 vs 24 months, p=0.004), and SHP2 expression level was identified as an independent prognostic factor for overall survival.

Conclusions:

  • SHP2 plays a significant role in NSCLC development and progression.
  • SHP2 has potential as a clinical biomarker for NSCLC prognosis.
Keywords:
Hook1SHP2non-small cell lung cancer (NSCLC)protein expression

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