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Updated: Jan 20, 2026
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
Published on: July 15, 2019
Novel method for action potential measurements from intact cardiac monolayers with multiwell microelectrode array
Heather B Hayes1, Anthony M Nicolini1, Colin A Arrowood1
1Axion Biosystems, Inc, Atlanta, GA, USA.
A new method called local extracellular action potential (LEAP) enables high-throughput, label-free measurement of cardiac action potentials from cardiomyocyte syncytium, improving drug testing and cardiac biology research.
Area of Science:
- Cardiology
- Biomedical Engineering
- Drug Discovery
Background:
- Cardiac action potential (AP) is crucial for cardiac health, disease, and drug safety.
- Existing high-throughput AP measurement techniques are limited.
Purpose of the Study:
- Introduce a novel, high-throughput method for measuring cardiac APs.
- Enhance the accuracy and automation of cardiac drug testing and stem cell model research.
Main Methods:
- Developed a technique to improve cardiomyocyte syncytium coupling to microelectrode arrays.
- Introduced local extracellular action potential (LEAP) for stable, label-free AP measurement.
- Quantified AP morphology using rise time, AP duration, beat period, and triangulation.
Main Results:
- Achieved reliable and stable LEAP measurements from intact cardiomyocyte syncytium.
- Demonstrated LEAP's efficacy in quantifying AP morphology in human iPSC-derived and rodent cardiomyocytes.
- Successfully quantified compound responses and genetic modification-induced AP changes.
Conclusions:
- LEAP is the first high-throughput, non-invasive, label-free method for capturing AP morphology from cardiomyocyte syncytium.
- LEAP can accelerate cardiac stem cell research and improve automated drug testing accuracy.
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