Related Experiment Video

Updated: Jan 20, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
09:29

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

18.8K

Long noncoding RNA LINC00511 promotes cell growth and invasion in triple-negative breast cancer by interacting with

Ruilei Liu1, Liang Wang2, Tianyu Gan1

  • 1Department of Thyroid and Breast Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

Cancer Management and Research
|August 17, 2019
PubMed

Insights

Long noncoding RNA LINC00511 is elevated in triple-negative breast cancer (TNBC). Downregulating LINC00511 inhibits TNBC cell growth and invasion, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant expression of long noncoding RNAs (lncRNAs) is implicated in various cancers.
  • Triple-negative breast cancer (TNBC) is an aggressive subtype with limited therapeutic options.
  • The role of specific lncRNAs, like LINC00511, in TNBC progression requires further investigation.

Purpose of the Study:

  • To investigate the expression and functional role of LINC00511 in triple-negative breast cancer (TNBC).
  • To explore the underlying molecular mechanisms by which LINC00511 influences TNBC progression.

Main Methods:

  • Quantitative reverse transcription PCR (RT-PCR) to assess LINC00511 expression in TNBC tissues and cell lines.
  • In vitro assays including MTT, colony formation, and Boyden assays to evaluate cell growth and invasion.
  • RNA pull-down and RNA immunoprecipitation (RIP) assays to identify proteins interacting with LINC00511.

Main Results:

  • LINC00511 expression was significantly upregulated in TNBC tissues compared to normal breast tissues.
  • Downregulation of LINC00511 suppressed TNBC cell proliferation and invasion.
  • LINC00511 was found to bind to the E3 ubiquitin ligase β-transducin repeat containing (BTRC).
  • LINC00511 stabilizes Snail by inhibiting its ubiquitination and degradation mediated by BTRC.

Conclusions:

  • LINC00511 plays a crucial role in promoting the progression of triple-negative breast cancer.
  • The LINC00511/BTRC/Snail axis represents a potential therapeutic target for TNBC treatment.
Keywords:
LINC00511Snailtriple-negative breast cancer

More Related Videos

Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish09:22

Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish

Published on: April 25, 2017

17.4K
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional 3D Model08:08

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional 3D Model

Published on: June 11, 2014

16.3K

Related Concept Videos

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.7K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.8K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.5K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.8K
Scalar and Vector Triple Products01:06

Scalar and Vector Triple Products

Two vectors can be multiplied using a scalar product or a vector product. The resultant of a scalar product is scalar, while with vector products, the resultant is a vector. These rules of the scalar or vector product between two vectors can be applied to multiple vectors to obtain meaningful combinations. The scalar triple product is the dot product of a vector with the cross product of two vectors.
The scalar triple product is the dot product of a vector with the cross product of two vectors....
4.4K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.3K