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Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes
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Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

Fei Deng1, Yi Li1, Md Jakir Hossain2

  • 1Graduate School of Biomedical Engineering, ARC Centre of Excellence in Nanoscale Biophotonics, Faculty of Engineering, UNSW Sydney, NSW 2052, Australia. guozhen.liu@unsw.edu.au and Australian Centre for NanoMedicine, UNSW Sydney, NSW 2052, Australia.

The Analyst
|August 17, 2019
PubMed

A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood. The substrate stainless steel (SS) was first coated with a polydopamine layer and subsequently grafted with poly(ethylene glycol) methacrylate brushes, onto which a sandwich immunosensor was modified for detection of IL-1β. The device has been successfully applied for monitoring of IL-1β with a limit of detection of 4.7 pg mL-1, and a linear detection range of 12.5-200 pg mL-1. Good specificity and selectivity for monitoring of IL-1β in rat macrophage secretion were achieved. Furthermore, this device was validated by detection of IL-1β in rat whole blood samples with greater concentrations observed in obese rats compared to control, and strong positive correlation between concentrations of IL-1β and blood glucose. These results suggest this device is feasible for direct detection of target analytes in biological samples.

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