Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.1K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.1K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

779
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
779
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

12.1K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
12.1K
What are Estimates?01:06

What are Estimates?

8.2K
It isn't easy to measure a parameter such as the mean height or the mean weight of a population. So, we draw samples from the population and calculate the mean height or mean weight of the individuals in the sample. This sample data acts as a representative measure of the population parameter. These sample statistics are known as estimates. 
The estimate for the mean of a sample is denoted by ͞x, whereas the mean of the population is designated as μ. Further, parameters such...
8.2K
Phase Diagrams02:39

Phase Diagrams

48.9K
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
48.9K
Structures of Solids02:22

Structures of Solids

17.5K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
17.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compact extended-DOF microscope through electrowetting lens.

Optics express·2025
Same author

Simulation of digital lensless holographic microscopy holograms: a physics-image processing approach.

Optics express·2025
Same author

Open-access database for digital lensless holographic microscopy and its application on the improvement of deep-learning-based autofocusing models.

Applied optics·2024
Same author

Management of the axial modulation of the illumination pattern in structured illumination microscopy using an extended illumination source.

Optics express·2023
Same author

Noise reduction in digital holography phase maps by phase-preserving discrete Fourier resampling.

Optics letters·2023
Same author

Focus Issue Introduction: 3D Image Acquisition and Display: Technology, Perception and Applications.

Optics express·2023
Same journal

Invaders taking over-Mollusc faunal change in volcanic barrier lakes of the Albertine Rift biodiversity hotspot.

PloS one·2026
Same journal

AI-driven molecular diversification and ligand-based optimization of macitentan derivatives targeting VEGFR1 and endothelin signaling pathways.

PloS one·2026
Same journal

Performance patterns and records in the world aquatics masters championships: Where do the most frequently represented nations among the top-ten masters swimmers come from?

PloS one·2026
Same journal

Modeling diurnal Temperature-Rainfall relationships under multicollinearity using PLS-SEM: A case study of Ghana.

PloS one·2026
Same journal

Organizational culture, social capital, and emergency capacity in primary healthcare institutions: A cross-sectional structural equation modeling study comparing ordinary and older communities.

PloS one·2026
Same journal

Impact of kidney function on the metabolome in the general population.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.4K

Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy.

Jorge Sola-Pikabea1, Arcadi Garcia-Rius1, Genaro Saavedra1

  • 1Department of Optics, 3D Imaging and Display Laboratory, Universitat of València, Valencia (Burjassot), Spain.

Plos One
|August 17, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a novel method for determining phase-shifts in two-dimensional Structured Illumination Microscopy (2D-SIM). The technique efficiently estimates phase-shifts by analyzing spectral components, improving accuracy and reducing computational load.

More Related Videos

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K
Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
07:53

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

7.7K

Related Experiment Videos

Last Updated: Jan 20, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.4K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K
Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
07:53

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

7.7K

Area of Science:

  • Optical Microscopy
  • Super-resolution Imaging
  • Image Processing

Background:

  • Accurate phase-shift determination is crucial for high-resolution imaging in 2D-SIM.
  • Existing methods for phase-shift estimation can be computationally intensive or less robust.
  • Uncertainty in phase-shifts degrades the quality of reconstructed super-resolution images.

Purpose of the Study:

  • To develop a fast and robust method for determining unknown phase-shifts in 2D-SIM.
  • To improve the accuracy of phase-shift estimation by analyzing spectral components.
  • To reduce the computational complexity compared to existing 2D-SIM phase-shift determination techniques.

Main Methods:

  • A novel method based on comparing peak intensities of spectral components in the Fourier domain.
  • Phase-shift estimation using the normalized peak intensity difference function.
  • Optimization algorithm for rapid phase-shift calculation.
  • Testing algorithm stability and robustness against noise and contrast variations.

Main Results:

  • The proposed method accurately determines phase-shifts in 2D-SIM.
  • The algorithm demonstrates robustness across various noise levels and pattern contrasts.
  • Significant reduction in computational requirements compared to other techniques.
  • Validation through theoretical calculations, simulations, and experimental data.

Conclusions:

  • The presented method offers an efficient and reliable approach for phase-shift determination in 2D-SIM.
  • This technique enhances the practical applicability of 2D-SIM by simplifying a critical calibration step.
  • The findings contribute to advancing super-resolution microscopy techniques through improved image reconstruction.