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Super-resolution Imaging of Proteus mirabilis Biofilm by Expansion Microscopy
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Expansion Microscopy: Scalable and Convenient Super-Resolution Microscopy.

Paul W Tillberg1, Fei Chen2

  • 1Janelia Research Campus, HHMI, Ashburn, Virginia 20147, USA;

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|August 20, 2019
PubMed
Summary
This summary is machine-generated.

Expansion microscopy (ExM) physically magnifies samples, enhancing microscope resolution for nanoscale studies. This technique offers scalable mapping and post-expansion molecular analysis, complementing live-cell imaging.

Keywords:
expansion microscopygel embeddingsuper-resolution imagingtissue expansion

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Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Microscopy resolution limits nanoscale visualization.
  • Expansion microscopy (ExM) offers a novel approach to overcome these limitations.

Purpose of the Study:

  • To describe the fundamental principles and recent variants of ExM.
  • To examine ExM applications in cell and developmental biology for nanoscale structure analysis.
  • To explore ExM's potential for scalable mapping and molecular interrogation.

Main Methods:

  • Physical magnification technique.
  • Hydrogel embedding and expansion protocols.
  • Post-expansion molecular analysis.

Main Results:

  • ExM significantly increases effective resolving power.
  • Demonstrated applications in studying nanoscale structures in cell and developmental biology.
  • Showcased potential for large-volume nanoscale mapping.
  • Enabled post-expansion molecular interrogation in a purified chemical environment.

Conclusions:

  • ExM is a versatile tool for nanoscale research.
  • ExM complements live-cell imaging techniques.
  • ExM is easily adopted, modified, and compatible with thick tissue specimens.