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Updated: Jan 20, 2026

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells
Published on: December 22, 2010
Synthesis and study of branched hyaluronic acid with potential anticancer activity
Radovan Buffa1, Kristina Nešporová1, Ivana Basarabová1
1Contipro ltd., Dolní Dobrouč 401, 56102, Czech Republic.
Abstract:
Conjugates of hyaluronic acid (HA) with biologically active molecules showed great potential in many applications. Oligomers of hyaluronic acid (oliHA) as possible regulators of biological processes and potential anti-cancer therapeutics, were reversibly attached via covalent bond on HA modified with an aldehyde group (HA-CHO) using bis-hydrazido or bis-oxyamino spacers. The structure of the final conjugates (HA-spacer-oliHA) was studied by advanced 2-dimensional NMR techniques in detail. The suggested synthetic strategy is simple, straightforward and can be used for the synthesis of various types of HA or oliHA conjugates. in vitro cytotoxicity assays showed selective activity of HA-spacer-oliHA conjugates against cancer cell lines in comparison with standard human fibroblasts.
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