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Updated: Jan 20, 2026

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
Published on: November 11, 2025
Oblique-plane single-molecule localization microscopy for tissues and small intact animals
Jeongmin Kim1,2, Michal Wojcik3, Yuan Wang1,2
1NSF Nanoscale Science and Engineering Center, University of California, Berkeley, Berkeley, CA, USA.
Abstract:
Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.
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