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Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma
Samuel White1,2,3, Meriel Moore-Colyer1, Eliane Marti4
1School of Equine Management and Science, Royal Agricultural University, Gloucestershire, United Kingdom.
Journal of Veterinary Internal Medicine
|August 21, 2019
Summary
A new protein microarray accurately profiles equine asthma (SEA) allergens, identifying novel triggers and confirming pollen sensitization. This technology aids in diagnosing and managing SEA in horses.
Area of Science:
- Equine immunology
- Allergen-specific IgE profiling
- Veterinary diagnostics
Background:
- Severe equine asthma (SEA) is a common, performance-limiting condition in horses.
- SEA is linked to increased immunoglobulin E (IgE) against environmental allergens.
Purpose of the Study:
- To develop a protein microarray for profiling IgE against environmental proteins in horses with SEA.
- To identify novel allergens contributing to SEA.
Main Methods:
- A 384-protein microarray was developed using diverse environmental proteins.
- Optimized printing, incubation, and immunolabeling techniques were assessed.
- Sera and bronchoalveolar lavage fluid (BALF) were analyzed for IgE.
Main Results:
- The platform identified novel allergens and confirmed a link between SEA and pollen sensitization.
- Assays demonstrated high accuracy (R²=0.91) and reproducibility (R²=0.97).
- Arthropods, fungi, and pollens were key discriminators for SEA.
Conclusions:
- Protein microarrays are effective for large-scale IgE mapping of equine allergens.
- This technology offers a platform for specific diagnosis, management, and treatment of SEA.
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