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Updated: Jan 20, 2026

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording
Published on: September 1, 2022
Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon
Gábor Orbán1,2, Domokos Meszéna2,3, Kinga Réka Tasnády2
1Doctoral School on Materials Sciences and Technologies, Óbuda University, Budapest, Hungary.
Abstract:
The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon microscopy around the implanted microelectrodes. A special filtering algorithm was used for data analysis to eliminate the artefacts caused by the imaging laser. Utilization of a special filtering algorithm allowed us to detect and sort single unit activities from simultaneous two-photon imaging and electrophysiological measurement.
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