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Phosphoglycerate mutase affects Stenotrophomonas maltophilia attachment to biotic and abiotic surfaces

Layla Ramos-Hegazy1, Shubham Chakravarty2, Gregory G Anderson1

  • 1Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN, USA.

Microbes and Infection
|August 21, 2019
PubMed

Insights

Stenotrophomonas maltophilia biofilm formation is a medical concern. A mutation in phosphoglycerate mutase (gpmA) impairs bacterial attachment, a key step in biofilm development.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Medical Science

Background:

  • Biofilm formation by Stenotrophomonas maltophilia poses a significant medical challenge, especially in lung infections.
  • The molecular underpinnings of S. maltophilia's biofilm lifestyle remain largely unelucidated.

Purpose of the Study:

  • To identify novel molecular factors influencing biofilm formation in Stenotrophomonas maltophilia.
  • To understand the role of specific genes in the initial stages of biofilm development.

Main Methods:

  • Generation and screening of a transposon mutant library of S. maltophilia.
  • Assessment of biofilm formation and surface attachment capabilities of wild-type and mutant strains.

Main Results:

  • A mutation in the glycolytic enzyme phosphoglycerate mutase (gpmA) gene was identified.
  • The gpmA mutant exhibited significantly impaired attachment to both abiotic and biotic surfaces compared to the wild type.

Conclusions:

  • Phosphoglycerate mutase (gpmA) plays a unique role in the initial adherence of S. maltophilia.
  • This finding offers a potential target for understanding and controlling S. maltophilia biofilm initiation and development.
Keywords:
BiofilmCystic fibrosisPhosphoglycerate mutaseStenotrophomonas maltophilia

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