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Mathematical models incorporating a multi-stage cell cycle replicate normally-hidden inherent synchronization in cell
Sean T Vittadello1, Scott W McCue1, Gency Gunasingh2
1School of Mathematical Sciences, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
Journal of the Royal Society, Interface
|August 22, 2019
Summary
Standard cell proliferation assays unintentionally synchronize cells, revealing hidden oscillatory subpopulations. This inherent synchronization impacts cell cycle studies and experimental reproducibility.
Area of Science:
- Cell Biology
- Biophysics
Background:
- Cell proliferation assays are fundamental in biological research.
- Standard methods aim to create asynchronous cell populations for reliable results.
Purpose of the Study:
- To investigate the presence of hidden synchronization in cell proliferation assays.
- To reconcile the apparent exponential growth with underlying oscillatory subpopulations.
Main Methods:
- Utilizing fluorescent cell cycle indicators to visualize cell cycle dynamics.
- Employing a multi-stage mathematical model to simulate cell proliferation.
Main Results:
- Standard cell proliferation assays exhibit persistent inherent cell synchronization.
- Fluorescent indicators reveal oscillatory subpopulations not visible in total population growth.
- Mathematical modeling successfully replicates observed oscillatory subpopulations.
Conclusions:
- Inherent cell synchronization is a previously unrecognized factor in standard assays.
- This synchronization can affect the reproducibility of cell cycle-dependent studies.
- Understanding inherent synchronization is crucial for improving experimental design and interpretation.
Keywords:
cell cyclecell proliferationfluorescent ubiquitination-based cell cycle indicatormathematical modelreproducibilitysynchronizationMore Related Videos
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