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Correction: cis or trans with class II diterpene cyclases.

Meirong Jia1, Reuben J Peters1

  • 1Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, IA 50010, USA. rjpeters@iastate.edu.

Organic & Biomolecular Chemistry
|August 22, 2019
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Summary

This correction clarifies the stereochemical outcome of reactions catalyzed by class II diterpene cyclases. It ensures accurate understanding of the cis and trans configurations in diterpene biosynthesis.

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Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Natural Product Synthesis

Background:

  • Class II diterpene cyclases are enzymes involved in the biosynthesis of diterpenoids.
  • Understanding the stereochemical control of these enzymes is crucial for elucidating natural product pathways.
  • Previous work required clarification regarding the cis/trans selectivity of these enzymes.

Purpose of the Study:

  • To correct and clarify the stereochemical assignments in a previously published study.
  • To provide accurate information on the reaction mechanisms of class II diterpene cyclases.

Main Methods:

  • Spectroscopic analysis (NMR, MS)
  • Chemical synthesis and characterization
  • Enzymatic assays

Main Results:

  • Correction of the stereochemical outcome for specific diterpene cyclization reactions.
  • Confirmation of the cis or trans selectivity influenced by class II diterpene cyclases.

Conclusions:

  • Accurate stereochemical data is essential for understanding diterpene biosynthesis.
  • This correction ensures the reliability of data concerning class II diterpene cyclases.