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Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
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Enhanced Subcellular Trafficking of Resveratrol Using Mitochondriotropic Liposomes in Cancer Cells

Ji Hee Kang1, Young Tag Ko2

  • 1College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon 21936, Korea.

Pharmaceutics
|August 23, 2019
PubMed

Insights

Novel mitochondria-targeting liposomes loaded with resveratrol demonstrate enhanced cancer cell death. These liposomes, TLS (Res) and DLS (Res), selectively accumulate in mitochondria, generating reactive oxygen species and disrupting membrane potential for effective cancer therapy.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Biology

Background:

  • Mitochondria are crucial organelles and a key target for cancer therapy.
  • Mitochondria-targeting strategies can overcome multi-drug resistance (MDR) in cancer cells.
  • Resveratrol exhibits anti-cancer properties but requires effective delivery.

Purpose of the Study:

  • To synthesize mitochondriotropic liposomes for targeted delivery of resveratrol.
  • To evaluate the efficacy of these liposomes in inducing cancer cell death via mitochondrial pathways.
  • To assess the potential of these liposomes in overcoming MDR mechanisms.

Main Methods:

  • Synthesis of TPP-DSPE-PEG and DQA-DSPE-PEG for mitochondriotropic liposomes.
  • Construction of resveratrol-loaded liposomes (TLS (Res) and DLS (Res)) with enhanced permeability and retention (EPR).
  • Confocal microscopy and LC-MS/MS to confirm cellular uptake and mitochondrial accumulation.

Main Results:

  • TLS (Res) and DLS (Res) liposomes exhibited ~120 nm size and positive zeta potential.
  • Demonstrated enhanced cellular uptake and selective mitochondrial accumulation of resveratrol.
  • Induced cancer cell cytotoxicity by generating reactive oxygen species (ROS) and dissipating mitochondrial membrane potential.

Conclusions:

  • Mitochondria-targeting liposomes (TLS (Res) and DLS (Res)) are effective for delivering resveratrol.
  • These liposomes offer a promising strategy for cancer treatment through mitochondrial targeting.
  • The approach stimulates mitochondrial signaling pathways for therapeutic benefit.
Keywords:
dequaliniumliposomesmitochondrial targetingresveratroltriphenylphosphonium cation

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