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Updated: Jan 20, 2026

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Published on: March 22, 2022

Targeted siRNA Delivery Using Lipid Nanoparticles.

Andreia Jorge¹, Alberto Pais², Carla Vitorino^3,4,5

¹Department of Chemistry, Coimbra Chemistry Centre, University of Coimbra, Coimbra, Portugal. andreiaj@qui.uc.pt.

Methods in Molecular Biology (Clifton, N.J.)

|August 23, 2019

Summary

This summary is machine-generated.

Solid lipid-based nanoparticles (SLNs) offer a promising solution for delivering small-interfering ribonucleic acid (siRNA) intracellularly. This review details SLN production methods and targeted delivery strategies to overcome clinical delivery challenges.

Keywords:

RNA interference Solid lipid-based nanoparticles Solvent-based methods Targeting Temperature-based methods siRNA delivery

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Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

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Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

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Published on: July 23, 2016

Area of Science:

Biomedical Engineering
Nanotechnology
Drug Delivery

Background:

Intracellular delivery of small-interfering ribonucleic acid (siRNA) remains a significant challenge for clinical applications.
siRNA therapeutics require efficient transport across biological barriers to reach target cells.
Solid lipid-based nanoparticles (SLNs) are explored as a potential delivery vehicle.

Purpose of the Study:

To provide a comprehensive overview of SLN production methods for siRNA delivery.
To critically appraise strategies for targeted siRNA delivery using SLNs.
To address the hurdles in clinical translation of siRNA therapeutics.

Main Methods:

Review of established and emerging SLN fabrication techniques.
Analysis of formulation design principles for optimal siRNA encapsulation and release.
Evaluation of targeting strategies for enhanced tissue-specific delivery.

Main Results:

SLNs can effectively encapsulate siRNA, protecting it from degradation.
Rational formulation and process design are crucial for efficient SLN production.
Targeting ligands can improve the specificity of siRNA delivery to desired organs or tissues.

Conclusions:

SLNs present a viable platform for overcoming intracellular delivery barriers for siRNA.
Optimized SLN formulations and targeted delivery approaches are key to advancing siRNA therapeutics.
Further research into SLN production and targeting is essential for clinical translation.