Related Experiment Video
Updated: Jan 20, 2026

Triplet Fusion Upconversion Nanocapsule Synthesis
Published on: September 7, 2022
Construction of a Macrophage-Targeting Bio-nanocapsule-Based Nanocarrier
Hao Li1, Masaharu Somiya1, Kenji Tatematsu1
1Department of Biomolecular Science and Reaction, The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan.
Abstract:
The construction protocol of bio-nanocapsule (BNC)-based nanocarriers, named GL-BNC and GL-virosome, for targeted drug delivery to macrophages is described here. First, genes encoding the Streptococcus sp. protein G-derived C2 domain (binds to IgG Fc) and Finegoldia magna protein L-derived B1 domain (binds to Igκ light chain) are prepared by PCR amplification. Subsequently, the genes encoding hepatic cell-specific binding domain of hepatitis B virus envelope L protein are replaced by these PCR products. The expression plasmid for this fused gene (encoding GL-fused L protein) can be used to transform Saccharomyces cerevisiae AH22R- cells. To obtain GL-BNC, the transformed yeast cells are disrupted with glass beads, treated with heat, and then subjected to IgG affinity column chromatography followed by size exclusion column chromatography. In addition, GL-BNCs can be fused with liposomes to form GL-virosome. The targeted delivery of GL-BNC and GL-virosome to macrophages can be confirmed by in vitro phagocytosis assays using the murine macrophage cell line RAW264.7.
More Related Videos
11:53Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
Published on: July 21, 2017
06:57Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation
Published on: August 11, 2018
Related Concept Videos
Bode Plots Construction
Construction of Root Locus
For positive gain values, the root locus exists on the real axis to the left of an odd number of finite open-loop poles or zeros. The root locus starts at the open-loop poles and traces the paths of the closed-loop poles as the gain...
Construction of Frequency Distribution
First, make a table with two columns—one with the title of the data that needs to be organized, and the other column for frequency. [Draw a third column for tally marks if needed]. Then, take a look at the items given in the data set and decide if an ungrouped frequency distribution table or a grouped frequency distribution table would be more suitable. If there are large sets of different values, then it is...
Design Example: Dimensioning of Concrete Masonry Construction
The site engineer has laid out a plan for the storeroom with external dimensions of twelve feet in length and...
Weak Base Solutions
Targeted Cancer Therapies
There are several types of targeted therapies against...