Related Experiment Video
Updated: Jan 20, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Efficient nitrogen removal in separate coupled-system of anammox and sulfur autotrophic denitrification with a
Tuo Wang1, Jianbo Guo1, Yuanyuan Song1
1School of Environmental and Municipal Engineering, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin Chengjian University, Jinjing Road 26, Tianjin 300384, China.
Abstract:
In order to achieve efficient nitrogen removal, a separate coupled-system of anaerobic ammonia oxidation (anammox) and sulfur autotrophic denitrification (S0-SADN) was established. In this study, the operational feasibility and stability of the coupled-system under substrate fluctuations were investigated. Results showed that the coupled-system improved the total nitrogen removal efficiency (TNRE) to 99.15 ± 0.68%. The tryptophan-like substances in anammox effluent positively impacted the growth of the S0-SADN biofilm. This positive cooperativity boosted the S0-SADN to achieve rapid 12-day startup and stable operation thereafter. The TNRE was determined at 95.27 ± 1.51% and 93.44 ± 0.96% under excessive nitrite and ammonium, respectively. The coupled-system recovered quickly after 21 days of starvation deterioration. To further treat the excessive ammonium, the nitrification side-branch of the coupled-system improved the TNRE to 99.08 ± 0.68%. Extracellular polymeric substances analysis revealed that the anammox and S0-SADN bacteria secreted protein-like substances to resist substrate fluctuation. Microbial community analysis indicated that the stability of bacterial community supported the stability of the coupled-system. These results collectively suggested that the separate coupled-system exhibited excellent performance and provided a platform for practical wastewater treatment in future.
Related Concept Videos
Chirality at Nitrogen, Phosphorus, and Sulfur
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
The Sulfur Cycle
The Nitrogen Cycle
Sulfur Assimilation
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Inorganic Nitrogen Assimilation

