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Long noncoding RNA SNHG16 silencing inhibits the aggressiveness of gastric cancer via upregulation of microRNA-628-3p

Weifeng Pang1, Mingcui Zhai2, Yue Wang3

  • 1Department of Internal Oncology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.

Cancer Management and Research
|August 27, 2019
PubMed
Abstract

Insights

Low microRNA-628-3p (miR-628) expression correlates with aggressive gastric cancer. Overexpressing miR-628 inhibits tumor growth, suggesting the SNHG16-miR-628-NRP1 pathway as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-628-3p (miR-628) is implicated in various cancers, but its role in gastric cancer is not well understood.
  • Investigating miR-628's expression profile and functional significance in gastric cancer progression is crucial.

Purpose of the Study:

  • To determine the expression levels of miR-628 in gastric cancer.
  • To elucidate the functional role and underlying mechanisms of miR-628 in gastric cancer aggressiveness.
  • To explore the potential of the miR-628 pathway as a therapeutic target.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for miR-628 expression analysis.
  • Cellular assays (CCK-8, flow cytometry, Transwell) and xenograft models to assess miR-628's functional impact.
  • Bioinformatics, luciferase reporter assays, RT-qPCR, and Western blotting to uncover molecular mechanisms.

Main Results:

  • Gastric cancer tissues and cell lines exhibit significantly lower miR-628 expression.
  • Low miR-628 expression is associated with advanced tumor stage, lymph node metastasis, and poorer patient prognosis.
  • Overexpression of miR-628 suppressed proliferation, migration, invasion, and tumor growth while inducing apoptosis in gastric cancer cells.
  • Neuropilin 1 (NRP1) was identified as a direct target, and long noncoding RNA SNHG16 acts as a sponge for miR-628.

Conclusions:

  • The SNHG16-miR-628-NRP1 axis is a key regulatory network in gastric cancer progression.
  • This pathway represents a promising novel therapeutic target for gastric cancer treatment.
Keywords:
gastric cancerlong noncoding RNAmicroRNA-628-3pneuropilin 1small nucleolar RNA host gene 16

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