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Related Concept Videos

Data Collection II01:29

Data Collection II

9.6K
The nursing history captures and records the patient's health status, so that a care plan evolves to meet the patient's individual needs. The nursing health history is a part of the initial assessment. A comprehensive history covers all health dimensions and plays a significant role in the assessment process. A comprehensive history includes the patient's biographical information, reasons for seeking health care, expectations, present and past health history, medications, and...
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Data Collection I01:30

Data Collection I

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Data collection gathers information needed to make accurate judgments about a patient's present condition. During a health history interview, subjective data is collected from the patient, their caregivers, or family members, and objective data is collected through observations and physical assessment. Patients are the primary source of subjective data. Thus information gathered from patients through interviews, observations, and physical examination is primary data. Secondary sources of...
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Data Collection by Experiments01:13

Data Collection by Experiments

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Data collection is a systematic method of obtaining, observing, measuring, and analyzing accurate information. An experimental study is a standard method of data collection that involves the manipulation of the samples by applying some form of treatment prior to data collection. It refers to manipulating one variable to determine its changes on another variable. The sample subjected to treatment is known as “experimental units.”
An example of the experimental method is a public...
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Data Collection by Survey01:07

Data Collection by Survey

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The systematic method of obtaining and analyzing accurate information of a population is called data collection. A survey is a standard method of data collection that involves collecting information from a target human population about their experience, opinion, or knowledge of a product, service, or process. The responses are recorded and interpreted. The most common survey examples are written questionnaires, face-to-face or telephonic conversations, focus groups, and electronic (e-mail or...
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Data Collection III01:05

Data Collection III

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The physical assessment examines the patient for objective data that defines the patient's condition, and aids in formulating the nursing care plan. The purpose of physical assessment is a health status appraisal, which includes identifying health problems, and establishing a database for nursing intervention.
The principles to begin the physical assessment include conducting a comprehensive or problem-related history in a quiet, well-lit room, emphasizing privacy and comfort for the...
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Data Collection by Observations01:08

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Data collection refers to a systematic way of obtaining, observing, measuring, and analyzing accurate information. Observational studies are one of the most widely used methods of data collection. It involves collecting data by observing the behavior and physical characteristics of a sample without making any modifications to the sample.
An astronomer viewing the motion and brightness of stars in the sky and recording the data is an example of observational data collection. A botanist recording...
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Counting Proteins in Single Cells with Addressable Droplet Microarrays
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On-Demand Droplet Collection for Capturing Single Cells.

Lang Nan1, Man Yuk Alison Lai2, Matthew Yuk Heng Tang1

  • 1Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, 999077, Hong Kong.

Small (Weinheim an Der Bergstrasse, Germany)
|August 27, 2019
PubMed
Summary
This summary is machine-generated.

This study presents an automated system for precise droplet collection, enabling efficient single-cell isolation and culture with over 95% recovery. This advances single-cell analysis by minimizing sample loss.

Keywords:
droplet microfluidicsquantitative collectionsingle cell

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Genomics

Background:

  • Droplet-based microfluidics are vital for single-cell analysis and understanding population heterogeneity.
  • Current methods face challenges in single-cell extraction from droplets, leading to sample loss.

Purpose of the Study:

  • To develop an automated system for accurate collection of droplets containing single cells.
  • To enable on-demand collection of single cells with high recovery rates.

Main Methods:

  • An automated system utilizing alternate sorting and dispensing in three branch channels for precise droplet control.
  • Integration of sorting for on-demand single-cell collection and a lossless recovery strategy.

Main Results:

  • Precise control of droplet numbers down to single-droplet resolution.
  • Achieved over 95% harvest rate for capture and culture of individual cells.
  • Demonstrated potential for quantitative processing and analysis of single cells.

Conclusions:

  • The developed on-demand droplet collection technique overcomes sample loss issues in single-cell isolation.
  • This method facilitates high-throughput, quantitative single-cell analysis.
  • It holds significant potential for elucidating cell-to-cell variations and their roles.