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Related Experiment Video

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Inducing Apical Periodontitis in Mice
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Inducing Apical Periodontitis in Mice.

Elisheva Goldman1, Eli Reich2, Itzhak Abramovitz3

  • 1Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University of Jerusalem; Department of Endodontics, Hadassah Ein Kerem Medical Center.

Journal of Visualized Experiments : Jove
|August 27, 2019
PubMed
Summary
This summary is machine-generated.

This study details a mouse model for inducing and studying apical periodontitis (AP), an inflammatory tooth root condition. The protocol involves exposing mouse dental pulp to oral flora, enabling analysis of inflammatory mechanisms in vivo.

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Area of Science:

  • Oral Biology
  • Inflammation Research
  • Animal Models

Background:

  • Apical periodontitis (AP) is a prevalent inflammatory condition affecting tissues around the tooth root.
  • Understanding the mechanisms of local induced inflammation is crucial in oral biology.
  • Existing animal models offer avenues for studying AP, but a standardized mouse protocol is beneficial.

Purpose of the Study:

  • To establish and demonstrate a reliable mouse model for inducing apical periodontitis (AP).
  • To provide a standardized in vivo experimental setting for studying the mechanisms of odontogenic inflammation.
  • To facilitate detailed characterization of AP using various analytical techniques.

Main Methods:

  • Induction of AP in mice by drilling into the tooth to expose the dental pulp.
  • Allowing natural oral flora contamination of the exposed pulp to trigger inflammation.
  • Sacrificing the animal for subsequent analysis of the tooth and jaw bone.

Main Results:

  • Successful induction of apical periodontitis (AP) in the mouse model.
  • Demonstration of a reproducible protocol for studying inflammatory processes in periodontal tissues.
  • Characterization of AP using techniques such as micro-CT, histology, immunohistochemistry, and RNA expression analysis.

Conclusions:

  • The described mouse model provides a valuable tool for in vivo research on apical periodontitis (AP) and related inflammatory mechanisms.
  • This standardized protocol allows for uniform experimental conditions and detailed analysis of AP.
  • The study highlights the importance of careful technique in handling animal models for oral biology research.