Identification and quantification of titanium nanoparticles in surface water: A case study in Lake Taihu, China

The accurate detection and quantification of nanoparticles (NPs) in aquatic environments are essential for toxicological and ecological risk assessment. Herein, we used single particle inductively coupled mass spectroscopy (SP-ICP-MS) to quantify titanium nanoparticles (Ti-NPs) in the extraction fractions of surface waters, and transmission electron microscopy coupled with an energy dispersive X-ray spectrometer (TEM-EDS) to specifically identify the nanoparticles. By using gold-NPs as reference standard, this approach achieved a Ti-NPs size detection limit in water of 25 nm with a particle number concentration limit of 10² particles/ml. We measured Ti-NPs concentrations in surface waters from Lake Taihu, China. The results revealed that the particles concentration was 2.78 × 10⁵ particles/ml with the mean size of 67 nm in October 2016, and the particles concentration of 2.28 × 10⁵ particles/ml with the mean size of 65 nm in April 2018, respectively. Based on TEM-EDS observation, various shapes of Ti-NPs were further identified, including regular cubes, long rods and flaky. We further measured the total organic carbon (TOC), and found that there was a positive correlation between Ti-NPs and TOC. This method enabled accurate detection and quantification of Ti-NPs concentration in environmental surface waters, which could be hugely useful for environmental risk assessment in aquatic systems.