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Updated: Jan 20, 2026

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
Published on: November 13, 2015
Construction strategy for ratiometric fluorescent probe based on Janus silica nanoparticles as a platform toward
Yang Xing1, Ying Zhou1, Li Fan2
1School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China.
Abstract:
Herein, we figured out a novel ratiometric fluorescent probe based on Janus silica nanoparticles (SiO2 NPs) where fluorescein isothiocyanate (FITC) and rhodamine B (RB) were asymmetrically functionalized for intracellular pH detection. With this unique strategy, the probe exhibited dual emission bands at 530 and 580 nm upon excitation at a single wavelength of 488 nm. The pH titrations of the probe showed a remarkable increase in ratiometric intensities ratio (I530nm/I580nm) when the pH was switched from 3.0 to 8.0 with a acid dissociation constant (pKa) value of 6.64. The probe was successfully applied to imaging and detection of pH in living cells without noticeable cytotoxicity, indicating that it is a promising candidate for bioanalysis and bioimaging.
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