PTPRO exaggerates inflammation in ulcerative colitis through TLR4/NF-κB pathway.

Area of Science:

• Immunology
• Gastroenterology
• Molecular Biology

Background:

• Ulcerative colitis (UC) is a chronic inflammatory bowel disease with incompletely understood mechanisms.
• Protein tyrosine phosphatase receptor type O (PTPRO) is implicated in inflammatory diseases, but its role in UC is unclear.

Purpose of the Study:

• To investigate the role and mechanism of PTPRO in ulcerative colitis (UC).
• To evaluate PTPRO as a potential diagnostic marker for UC.

Main Methods:

• Analysis of PTPRO, TLR4, and cytokine expression in UC patient tissues and cells.
• In vitro studies using lipopolysaccharide (LPS)-induced macrophage models with PTPRO manipulation.
• Assessment of intestinal epithelial cell (IEC) proliferation and apoptosis.
• Evaluation of NF-κB signaling pathway activation.
• Receiver operating characteristic (ROC) curve analysis for diagnostic potential.

Main Results:

• PTPRO, TLR4, and inflammatory cytokine expression were elevated in UC tissues and cells.
• PTPRO promoted TLR4 and cytokine expression in LPS-induced macrophages.
• PTPRO inhibited IEC proliferation and enhanced IEC apoptosis.
• PTPRO overexpression increased phosphorylated NF-κB/p65 and IκBα levels.
• PTPRO demonstrated significant diagnostic potential for UC (AUC = 0.807).

Conclusions:

• PTPRO exacerbates UC inflammation through the TLR4/NF-κB signaling pathway.
• PTPRO represents a promising diagnostic biomarker for ulcerative colitis.