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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.3K
Adult Stem Cells01:33

Adult Stem Cells

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

4.5K
A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
4.5K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

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Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
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Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

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Cancer stem cell induction from mouse embryonic stem cells.

Akimasa Seno1,2, Chikae Murakami3, Bishoy El-Aarag1,4

  • 1Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, Japan.

Oncology Letters
|August 28, 2019
PubMed
Summary

Mouse embryonic stem cells (mESCs) can be converted into cancer stem cells (CSCs) using conditioned medium from cancer cells. This finding expands the potential sources for CSC generation beyond induced pluripotent stem cells (iPSCs).

Keywords:
cancer stem cellconditioned mediummouse embryonic stem cells

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Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro
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Area of Science:

  • Stem cell biology
  • Cancer research
  • Cellular reprogramming

Background:

  • Cancer recurrence is often due to resistant cancer stem cells (CSCs).
  • Investigating CSCs is challenging due to their low numbers in tumors.
  • Previous work showed induced pluripotent stem cells (iPSCs) can generate CSCs.

Purpose of the Study:

  • To investigate if mouse embryonic stem cells (mESCs) can be converted into CSCs.
  • To determine if factors in cancer cell conditioned medium induce CSCs.
  • To explore the potential of mESCs in CSC generation.

Main Methods:

  • Culture of mouse embryonic stem cells (mESCs).
  • Treatment of mESCs with conditioned medium from cancer cells.
  • Analysis of converted cells for CSC properties.

Main Results:

  • Mouse embryonic stem cells (mESCs) were successfully converted into cancer stem cells (mES-CSCs).
  • This conversion occurred without gene editing, suggesting conditioned medium contains inductive factors.
  • The ability to generate CSCs is not exclusive to iPSCs, highlighting stem cell pluripotency.

Conclusions:

  • Embryonic stem cells, like mESCs, can be reprogrammed into CSCs.
  • Cancer cell-derived factors in conditioned media play a role in CSC induction.
  • mES-CSCs offer a new model for studying cancer mechanisms and drug screening.