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Updated: Jan 20, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
Published on: August 15, 2014
Quantitative discrimination of biological tissues by micro-elastographic measurement using an epi-illumination
Pradipta Mukherjee1, Tomohisa Horiguchi2, Shuhei Shibata1
1Graduate School of Engineering, Utsunomiya University, Utsunomiya, Tochigi, 321-8585, Japan.
Abstract:
We propose a method for estimating the stiffness of bio-specimens by measuring their linear retardance properties under applied stress. For this purpose, we employ an epi-illumination Mueller matrix microscope and show the procedures for its calibration. We provide experimental results demonstrating how to apply Mueller matrix data to elastography, using chicken liver and chicken heart as biological samples. Finally, we show how the histograms of linear retardance images can be used to distinguish between specimens and quantify the discrimination accuracy.
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