Related Experiment Video
Updated: Jan 20, 2026

Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
Published on: March 14, 2019
Establishment of a Drosophila AD model
Xingjun Wang1, Yu Zhao1, Yujia Hu1
1Institute of Intervention Vessel, Shanghai 10 People's Hospital, Shanghai Key Laboratory of Signaling and Diseases Research, School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Abstract:
Alzheimer's disease (AD) is the most common form of dementia that affects people's health greatly. Though amyloid precursor protein (APP) has been implicated in the pathogenesis of AD, the exact role of APP and its underlying mechanism in AD progression have remained largely elusive. Drosophila melanogaster has been extensively used as a model organism to study a wide range of human diseases including AD. In this protocol, we expressed full length human APP in the Drosophila nervous system and examined its effect on locomotion and choice ability. We found that expression of APP produced locomotion defects in larvae as measured by plate crawling ability assay (PCA), and in adult flies as monitored by plate cycling ability assay (CLA). In addition, expression of APP results in male courtship choice (MCC) defect, since wild-type males court preferentially toward young virgin females over old ones, while APP-expressing males failed to show this preference. This protocol enables us to screen for novel AD candidate genes as well as therapeutic compounds to ameliorate the disease.
Related Concept Videos
Clinically Relevant Drug Product Specifications: Methods of Establishment
Molecular Models
The Bohr Model
Stereotype Content Model
Clearance Models: Physiological Models
The organ's clearance rate depends on the blood flow to the organ and the extraction ratio (E). The extraction ratio describes the organ's...
Clearance Models: Compartment Models

