Related Experiment Video

Updated: Jan 20, 2026

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
07:36

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Published on: June 25, 2021

8.0K

Correlation between immunohistochemistry and RICTOR fluorescence in situ hybridization amplification in small cell

Ildiko Krencz1, Anna Sebestyen1, Judit Papay1

  • 11st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary H-1085.

Human Pathology
|August 28, 2019
PubMed

Insights

Immunohistochemistry (IHC) for Rictor protein is a reliable and cost-effective method to identify small cell lung cancer (SCLC) patients with RICTOR gene amplification, aiding in selecting candidates for targeted therapies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Small cell lung carcinoma (SCLC) is a challenging cancer with limited targeted therapy options.
  • The RICTOR gene, encoding a component of mTOR complex 2, is frequently amplified in SCLC and represents a potential therapeutic target.

Purpose of the Study:

  • To compare immunohistochemical (IHC) expression of Rictor and phospho-Akt with RICTOR gene amplification in SCLC.
  • To evaluate Rictor IHC as a potential surrogate for RICTOR amplification detection.

Main Methods:

  • 100 SCLC samples underwent RICTOR fluorescence in situ hybridization (FISH) and Rictor/phospho-Akt IHC staining.
  • FISH was used as the gold standard for RICTOR amplification detection.

Main Results:

  • RICTOR amplification was found in 15% of SCLC samples.
  • Rictor IHC showed high sensitivity (93%) and specificity (73%) for detecting RICTOR amplification.
  • High Rictor or phospho-Akt expression correlated with decreased overall survival, but RICTOR amplification did not correlate with clinical outcome.

Conclusions:

  • Rictor IHC expression strongly correlates with RICTOR amplification in SCLC.
  • Rictor IHC serves as a cost-effective method for patient selection for RICTOR FISH and potential mTORC1/2 inhibitor therapy.
Keywords:
Fluorescence in situ hybridizationImmunohistochemistryPhospho-AktRICTORSmall cell lung carcinoma

More Related Videos

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology13:05

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

27.5K
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification14:21

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

Published on: October 19, 2017

13.7K

Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
23.9K
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
10.4K
Correlations02:20

Correlations

Correlation means that there is a relationship between two or more variables (such as ice cream consumption and crime), but this relationship does not necessarily imply cause and effect. When two variables are correlated, it simply means that as one variable changes, so does the other. We can measure correlation by calculating a statistic known as a correlation coefficient. A correlation coefficient is a number from -1 to +1 that indicates the strength and direction of the relationship between...
35.8K
Correlation and Causation01:27

Correlation and Causation

Statistical tests can calculate whether there is a relationship, or correlation, between independent and dependent variables. An indirect relationship of the variables signifies a correlation, while a direct relationship shows causation. If it is determined that no connection exists between the variables, then the correlation is a coincidence.
Correlation versus Causation
If the dependent variable increases or decreases when the independent variable increases, there is a positive or negative...
42.0K
Hybrid Zones02:29

Hybrid Zones

Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
21.8K
Correlation01:09

Correlation

In statistics, two variables are said to be correlated if the values of one variable are associated with the other variable. Depending on the relationship between two variables, correlation can be of three types– positive correlation, negative correlation, and zero correlation.
Two variables, for example, a and b, are said to be positively correlated if both variables move in the same direction. In other words, a positive correlation exists between two variables, a and b, if:
14.8K