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Updated: Jan 20, 2026

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
Published on: March 23, 2022
Generation of integration free human iPSC line SORMi002-A from neonatal foreskin fibroblasts
Akshatha Shivaraj1, Shagufta Parveen1
1Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, GKVK post, Bellary Road, Bengaluru, Karnataka 560065, India.
Abstract:
Circumcision is a common surgical procedure usually performed on neonates worldwide. Skin removed after circumcision is abundant and often discarded. Neonatal foreskin fibroblasts (NFFs) can be easily isolated from it for application in biomedical research including induced pluripotent stem cell (iPSC) generation. In this study NFFs were reprogrammed to iPSCs using non-integrating episomal reprogramming plasmids. The iPSCs generated express pluripotency markers. They form embryoid bodies and differentiate in vitro into cells of endoderm, mesoderm and ectoderm. They have a normal male karyotype, are genetically identical to the parent fibroblasts and are transgene free.
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