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Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
Published on: July 30, 2020
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Tunable Surface Properties of Temperature-Responsive Polymer-Modified Liposomes Induce Faster Cellular Uptake
Jian Wang1, Eri Ayano1, Yoshie Maitani1
1Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato, Tokyo 105-8512, Japan.
ACS Omega
|August 29, 2019
Summary
Temperature-responsive liposomes modified with a novel copolymer enhance intracellular drug delivery. These advanced carriers show improved cellular uptake and drug release above body temperature, overcoming previous limitations.
Area of Science:
- Biotechnology
- Materials Science
- Nanomedicine
Background:
- Intracellular drug delivery using nanoparticle carriers faces challenges with efficiency.
- Temperature-responsive liposomes offer potential for controlled drug release upon heating.
Purpose of the Study:
- To synthesize and characterize a novel temperature-responsive copolymer, P(NIPAAm-co-DMAPAAm).
- To investigate the properties of liposomes modified with this copolymer for enhanced intracellular drug delivery.
Main Methods:
- Synthesis of poly(N-isopropylacrylamide)-co-N,N'-dimethylaminopropylacrylamide (P(NIPAAm-co-DMAPAAm)).
- Modification of liposomes with the synthesized copolymer.
- Evaluation of liposome aggregation, drug release, and fixed aqueous layer thickness (FALT) at varying temperatures.
- Comparison of cellular uptake and cytosolic release with PEGylated liposomes above 37 °C.
Main Results:
- The copolymer exhibited a lower critical solution temperature (LCST) above body temperature.
- Temperature-responsive liposomes aggregated and released content above the LCST.
- Liposome FALT decreased with increasing temperature, indicating surface dehydration.
- Cytosolic release and cellular uptake were significantly higher for modified liposomes compared to PEGylated liposomes above 37 °C.
Conclusions:
- Tunable surface properties of the temperature-responsive polymer-modified liposomes facilitate dehydration upon heating.
- This dehydration likely enhances cellular uptake and drug release kinetics.
- These liposomes show significant potential for improving intracellular drug delivery applications.
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