Related Experiment Video
Updated: Jan 20, 2026
Ultraviolet-Visible UV-Vis Spectroscopy: Principle and Uses
Published on: April 30, 2023
3D Printed UV-Visible Cuvette Adapter for Low-Cost and Versatile Spectroscopic Experiments
Heather D Whitehead1, Julia V Waldman1, Denise M Wirth1
1Department of Chemistry and Biochemistry, The University of Tulsa, 800 S. Tucker Dr., Tulsa, Oklahoma 74104, United States.
Abstract:
Ultraviolet-visible (UV-vis) spectroscopy represents one of the most popular analytical techniques in chemical research labs. A variety of vendors provide instruments that are suited for the analysis of liquid samples at moderate concentrations. However, to accommodate more specialized experiments, expensive accessories are required and often do not fit the specific needs of experimental scientists. In this work, we present a generalized adapter that can be 3D printed and used with existing spectrometers to enable a wide array of experiments to be performed. In the case of liquid samples, we provide a method for dramatically reducing the price of a quartz cuvette with minimal impact on performance. Through simple modification of the design, cuvettes with various path lengths can be prepared. Additionally, we illustrate the ability to turn any sample container into a working cuvette to simplify experimental protocols, prevent contamination risks, and further reduce costs. This strategy also enables gaseous and solid samples to be evaluated easily and reproducibly. Furthermore, we demonstrate how this concept can be extended to interface additional instrumentation with a commercial UV-vis spectrometer. All of the digital designs are provided under a creative commons license to enable other researchers to modify and adapt the designs for their unique experimental requirements.
Related Concept Videos
Ultraviolet-Visible (UV-Vis) Spectroscopy
Ultraviolet-visible (UV-Vis) spectroscopy is one of the most popular analytical techniques because it is very versatile and able to detect nearly every molecule. With UV-Vis spectroscopy, the UV-Vis light is passed through a sample and the transmittance of light by a sample is measured. From the transmittance (T), the absorbance can be calculated as A=-log (T). An absorbance spectrum is obtained that shows the absorbance of a...
05:16UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
06:443D Printing - Evaluating Particle Emissions of a 3D Printing Pen
10:43Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding
08:28Stereolithographic 3D Printing with Renewable Acrylates
11:45Creation of a High-Fidelity, Low-Cost, Intraosseous Line Placement Task Trainer via 3D Printing

