Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rapid Development of Cell State Identification Circuits with Poly-Transfection09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

2.0K
Complex genetic circuits are time-consuming to design, test, and optimize. To facilitate this process, mammalian cells are transfected in a way that allows the testing of multiple stoichiometries of circuit components in a single well. This protocol outlines the steps for experimental planning, transfection, and data analysis.
2.0K
Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification08:51

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

10.2K
A protocol for the preparation of poly(pentafluorophenyl acrylate) (poly(PFPA)) grafted silica beads is presented. The poly(PFPA) functionalized surface is then immobilized with antibodies and used successfully for the protein separation through...
10.2K
Synthesis of Terpolymers at Mild Temperatures Using Dynamic Sulfur Bonds in Poly(S-Divinylbenzene)09:16

Synthesis of Terpolymers at Mild Temperatures Using Dynamic Sulfur Bonds in Poly(S-Divinylbenzene)

8.2K
The goal of this protocol is to initiate polymerization using dynamic sulfur bonds in poly(S-divinylbenzene) at mild temperatures (90 °C) without using solvents. Terpolymers are characterized by GPC, DSC and 1H NMR, and tested for changes in...
8.2K
Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering12:22

Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

12.4K
An injectable tissue engineering scaffold composed of poly(N-isopropylacrylamide)-graft-chondroitin sulfate (PNIPAAm-g-CS)-containing alginate microparticles was prepared. The adhesive strength, swelling properties and in vitro biocompatibility are analyzed in this study. The characterization techniques developed here may be applicable to other thermogelling...
12.4K
Measurement of Poly A Tail Length from Drosophila Larva Brain and Cell Line08:16

Measurement of Poly A Tail Length from Drosophila Larva Brain and Cell Line

1.4K
The protocol describes an efficient and reliable method for quantifying the poly(A) length of the gene of interest from the Drosophila nervous system, which can be easily adapted to tissues or cell types from other species.
1.4K
A Simple and Efficient Protocol for the Catalytic Insertion Polymerization of Functional Norbornenes09:08

A Simple and Efficient Protocol for the Catalytic Insertion Polymerization of Functional Norbornenes

11.0K
We describe the catalytic insertion polymerization of 5-norbornene-2-carboxylic acid and 5-vinyl-2-norbornene to form functional polymers with a very high glass transition temperature.
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patterns in topical treatment use for eczema flare-ups in children: a secondary analysis of the Best Emollients for Eczema (BEE) trial.

Skin health and disease·2026
Same author

Metastatic Colorectal Cancer as a Chronic Disease: Twelve-Year Survival After Initially Unresectable Bilobar Liver Metastases.

Cureus·2026
Same author

RNAi-based acaricides: On the Path to commercialization of new tools for mite management in crops and hives.

Insect biochemistry and molecular biology·2026
Same author

Intracerebral Hemorrhage With Superimposed Psychiatric Illness: Navigating the Evaluation of Altered Mental Status.

Cureus·2026
Same author

O03 Rapid eczema trials: generating evidence that patients need.

The British journal of dermatology·2025
Same author

Development and validation of an LC-MS/MS method for the quantification of novel therapeutic TT-478, a selective adenosine receptor 2B antagonist, for a phase I/II clinical trial.

Bioanalysis·2025

Related Experiment Video

Updated: Jan 20, 2026

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

2.0K

Sugar-Functional Vinyl Addition Poly(norbornene)-Photopatternable Poly(norbornenyl gluconamide) Compositions

Steven Smith1, Liladhar Paudel1, Crystal Cyrus1

  • 1Promerus, LLC, 9921 Brecksville Road, Brecksville, Ohio 44141, United States.

ACS Omega
|August 29, 2019
PubMed
Summary

Vinyl addition polymerization of norbornenyl gluconamide (NBGA) yields water-soluble polymers. These polymers can be cross-linked and photopatterned, offering versatile applications in materials science.

More Related Videos

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

10.2K
Synthesis of Terpolymers at Mild Temperatures Using Dynamic Sulfur Bonds in PolyS-Divinylbenzene
09:16

Synthesis of Terpolymers at Mild Temperatures Using Dynamic Sulfur Bonds in PolyS-Divinylbenzene

Published on: May 20, 2019

8.2K

Related Experiment Videos

Last Updated: Jan 20, 2026

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

2.0K
Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

10.2K
Synthesis of Terpolymers at Mild Temperatures Using Dynamic Sulfur Bonds in PolyS-Divinylbenzene
09:16

Synthesis of Terpolymers at Mild Temperatures Using Dynamic Sulfur Bonds in PolyS-Divinylbenzene

Published on: May 20, 2019

8.2K

Area of Science:

  • Polymer Chemistry
  • Organic Synthesis
  • Materials Science

Background:

  • Norbornenyl gluconamide (NBGA) is a monomer with potential for creating functional polymers.
  • Vinyl addition polymerization offers a route to controlled polymer architectures.
  • Water-soluble polymers are desirable for various applications, including photolithography.

Purpose of the Study:

  • To investigate the vinyl addition polymerization of NBGA using palladium catalysts.
  • To characterize the resulting poly(NBGA) and explore its properties, including cross-linking and photopatterning.
  • To analyze the stereochemistry of the NBGA monomer synthesis.

Main Methods:

  • Palladium-catalyzed vinyl addition polymerization of NBGA.
  • Thermal cross-linking of poly(NBGA) with glutaraldehyde.
  • Formation of photopatternable compositions with diazides.
  • High-resolution nuclear magnetic resonance (NMR) spectroscopy for monomer analysis.

Main Results:

  • High-molecular-weight, water-soluble poly(NBGA) was successfully synthesized.
  • Poly(NBGA) demonstrated thermal cross-linking capabilities at elevated temperatures.
  • Photopatternable compositions were formed, enabling negative-tone imaging via water development.
  • NMR analysis revealed the formation of diastereomers in NBGA monomer synthesis.

Conclusions:

  • Palladium-catalyzed polymerization provides a viable route to water-soluble poly(NBGA).
  • Poly(NBGA) exhibits tunable properties for thermal cross-linking and photopatterning applications.
  • Understanding NBGA monomer stereochemistry is crucial for polymer synthesis control.