Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

Nucleic Acids

Nucleic Acids

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of...

Surface Properties of Synthesized Nanoporous Carbon and Silica Matrices

Surface Properties of Synthesized Nanoporous Carbon and Silica Matrices

Here we report the synthesis and characterization of ordered nanoporous carbon (with a 4.6 nm pore size) and SBA-15 (with a 5.3 nm pore size). The work describes the surface and textural properties of nanoporous molecular sieves, their wettability, and the melting behavior of D2O confined in the...

Isolating Nucleic Acids from Yeast

Isolating Nucleic Acids from Yeast

One of the many advantages to using yeast as a model system is that large quantities of biomacromolecules, including nucleic acids (DNA and RNA), can be purified from the cultured cells.
This video will address the steps required to carry out nucleic acid extraction. We will begin by briefly outlining the growth and harvest, and lysis of yeast cells, which are the initial steps common to the isolation of all biomacromolecules. Next, we will discuss two unique purification methods for the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Stimuli-Responsive Polyethylenimine Derivatives as Carriers for siRNA Delivery: Current Solutions and Future Perspectives.

ACS omega·2026

Same author

Inhibiting biofilm growth on ammonium salt-functionalized or fluorinated voice prostheses silicone.

Applied microbiology and biotechnology·2026

Same author

Unlocking Lewis acid-free metalation of O-nucleophiles in HFIP.

Communications chemistry·2026

Same author

Towards the design of ionic traps incorporating thermolabile units: structural characterization of copper(II), palladium(II) and rhodium(II) complexes with the aminoalcohol ligand 2-(pyridin-2-ylamino)ethanol.

Acta crystallographica. Section C, Structural chemistry·2026

Same author

Flame-Retardant and Hydrophobic Cotton via Alkoxysilyl-Functionalized Polysiloxanes, Cyclosiloxanes, and POSS with Surface Thiol-Ene Dithiophosphate Grafting.

Materials (Basel, Switzerland)·2026

Same author

Triazine-based cobalt pincer complex for regioselective C-H borylation of furans.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: Jan 20, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Silica Surface Modification and Its Application in Permanent Link with Nucleic Acids.

Krzysztof Kuciński¹, Magdalena Jankowska-Wajda¹, Tomasz Ratajczak²

¹Faculty of Chemistry, Adam Mickiewicz University in Poznań, Umultowska 89b, 61-614 Poznań, Poland.

|August 29, 2019

Summary

Platinum-catalyzed hydrosilylation created novel alkoxysilanes for silica surface modification. These modified surfaces effectively anchored nucleic acids for hybridization detection using fluorescence microscopy.

More Related Videos

Nucleotides and Nucleic Acids

Nucleotides and Nucleic Acids

Nucleic Acid Chemistry: DNA and RNA

Nucleic Acid Chemistry: DNA and RNA

Related Experiment Videos

Last Updated: Jan 20, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Nucleotides and Nucleic Acids

Nucleotides and Nucleic Acids

Nucleic Acid Chemistry: DNA and RNA

Nucleic Acid Chemistry: DNA and RNA

Area of Science:

Materials Science
Organic Chemistry
Biochemistry

Background:

Silica surfaces are widely used as solid supports in various applications.
Efficient methods for functionalizing silica surfaces are crucial for immobilizing biomolecules.
Nucleic acid immobilization is essential for applications like diagnostics and solid-phase synthesis.

Purpose of the Study:

To develop a novel method for modifying silica surfaces using platinum-catalyzed hydrosilylation.
To synthesize and apply bifunctional alkoxysilanes for O-silylation of silica.
To utilize the modified silica as a support for nucleic acid immobilization and detection.

Main Methods:

Platinum-catalyzed hydrosilylation of hydroxyl ethers to synthesize bifunctional alkoxysilanes.
O-silylation of silica surfaces with the synthesized alkoxysilanes.
Covalent linking of synthetic nucleic acids to the modified silica support.
Hybridization of immobilized nucleic acids with complementary, fluorescence-labeled sequences.
Detection of hybridized sequences using fluorescence microscopy.

Main Results:

Successful synthesis of various bifunctional alkoxysilanes.
Effective O-silylation of silica surfaces, creating functionalized supports.
Stable immobilization of nucleic acids onto the modified silica.
Successful hybridization of immobilized nucleic acids with complementary sequences.
Sensitive detection of anchored nucleic acids via fluorescence microscopy.

Conclusions:

Pt-catalyzed hydrosilylation provides an efficient route to functionalize silica surfaces.
The developed method allows for robust nucleic acid immobilization on solid supports.
This approach is suitable for applications requiring nucleic acid detection, such as in diagnostics or research.