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Array-Based Protein Sensing Using an Aggregation-Induced Emission (AIE) Light-Up Probe.

Huyeon Choi1, Sangpil Kim1, Seungho Lee1

  • 1Department of Chemistry, Ulsan National Institute of Science and Technology, UNIST-gil 50, Ulsan 44919, South Korea.

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|August 29, 2019
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Summary
This summary is machine-generated.

This study introduces a novel "turn on" fluorescent sensor for protein detection and identification. The aggregation-induced emission luminogens (AIEgens) platform offers a facile method for diagnosing diseases by distinguishing specific proteins.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Protein detection is crucial for disease diagnosis, but developing simple sensing probes remains a challenge.
  • Aggregation-induced emission luminogens (AIEgens) offer unique optical properties for sensing applications.

Purpose of the Study:

  • To develop a facile, array-based "turn on" protein-sensing platform using AIEgens.
  • To enable sensitive detection and identification of proteins for diagnostic purposes.

Main Methods:

  • Utilized water-soluble AIEgens that exhibit "turn on" fluorescence upon protein binding.
  • Investigated the influence of chemical functional groups on AIEgen-protein binding affinities.
  • Employed linear discriminant analysis for protein discrimination based on fluorescence outputs.

Main Results:

  • Achieved "turn on" fluorescence in the presence of nanomolar protein concentrations by restricting AIEgen intramolecular rotation.
  • Demonstrated distinct fluorescence signals for different proteins based on AIEgen binding affinities.
  • Successfully detected and discriminated target proteins and classified their concentrations using the array-based sensor.

Conclusions:

  • AIEgens serve as effective sensing probes in array-based systems for protein detection and identification.
  • The developed platform provides a novel approach for sensitive and specific protein analysis.
  • This technology holds promise for advancing disease diagnosis through facile protein sensing.