Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

8.4K
A reliable and easily reproducible method for preparation of functionalizable, near-infrared emitting photoluminescent gold nanoclusters and their direct detection inside HeLa cells by flow cytometry and confocal laser scanning microscopy is...
8.4K
Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion10:30

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

10.0K
Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are...
10.0K
RNA Stability01:53

RNA Stability

35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K
Emission Spectra02:39

Emission Spectra

75.7K
When solids, liquids, or condensed gases are heated sufficiently, they radiate some of the excess energy as light. Photons produced in this manner have a range of energies, and thereby produce a continuous spectrum in which an unbroken series of wavelengths is present.
75.7K
Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution10:27

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

6.6K
Dual DNA ruler assay is developed to determine the mRNA position during ribosome translocation, which relies on the dissociation forces of the formed DNA-mRNA duplexes. With single-nucleotide resolution and capability of reaching both ends of mRNA, it can provide mechanistic insights for ribosome translocation and probe other nucleic acid...
6.6K
Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride07:23

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride

14.9K
This paper aims to present a method to form smooth and well-controlled films of silver chloride (AgCl) with designated coverage on top of thin film silver...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two of a Kind: Composition and Photophysics of Two Silver Nanoclusters Stabilized by the Same DNA Sequence.

Journal of the American Chemical Society·2026
Same author

A Mixed-Valent and High-Spin Vanadium Phosphide.

Journal of the American Chemical Society·2026
Same author

Real-Time In Vivo Detection of Nanocarrier Number and Velocity in the Cerebrovasculature Using Hot Band Absorption.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Insights into the crystal packing interactions of a 960-nm-emissive DNA-stabilized silver nanocluster.

Chemical communications (Cambridge, England)·2026
Same author

Water-Based Synthesis of Supported Ultrasmall High-Entropy Alloy Nanoparticles.

Nano letters·2025
Same author

Dynamic Equilibria between DNA-Stabilized Silver Nanoclusters and Silver-Carrying DNA Strands.

The journal of physical chemistry letters·2025

Related Experiment Video

Updated: Jan 20, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

8.4K

Switchable Dual-Emissive DNA-Stabilized Silver Nanoclusters.

Cecilia Cerretani1, Tom Vosch1

  • 1Nano-Science Center/Department of Chemistry, University of Copenhagen. Universitetsparken 5, 2100 Copenhagen, Denmark.

ACS Omega
|August 29, 2019
PubMed
Summary

Researchers developed a DNA-silver nanocluster (DNA-AgNC) that reversibly switches between red and green emission. This switchable DNA-AgNC system, triggered by hydrogen peroxide and sodium borohydride, shows potential for novel ratiometric sensors.

More Related Videos

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion
10:30

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

Published on: September 4, 2013

10.0K
Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
10:27

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

6.6K

Related Experiment Videos

Last Updated: Jan 20, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

8.4K
Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion
10:30

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

Published on: September 4, 2013

10.0K
Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
10:27

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

6.6K

Area of Science:

  • Nanomaterials Science
  • Bioconjugate Chemistry
  • Photophysics

Background:

  • Silver nanoclusters (AgNCs) stabilized by single-stranded DNA (ss-DNA) exhibit unique photoluminescent properties.
  • Controlling the emission color and stability of DNA-AgNCs is crucial for their application in sensing and imaging.
  • Reversible switching between different emission states in nanomaterials is a highly sought-after feature.

Purpose of the Study:

  • To investigate an ss-DNA sequence capable of stabilizing both red- and green-emissive silver nanoclusters (DNA-AgNCs).
  • To demonstrate and characterize the reversible interconversion between red and green emissive states of the DNA-AgNCs.
  • To explore the potential of this switchable DNA-AgNC system for developing ratiometric sensors.

Main Methods:

  • Synthesis and characterization of DNA-stabilized silver nanoclusters (DNA-AgNCs).
  • Photophysical characterization including steady-state emission, quantum yield, fluorescence lifetime, and anisotropy measurements.
  • Investigating the reversible color switching triggered by chemical agents like hydrogen peroxide (H2O2) and sodium borohydride (NaBH4).

Main Results:

  • An ss-DNA sequence was identified that stabilizes both red- and green-emissive AgNCs.
  • A reversible switching mechanism between red and green emission was demonstrated, triggered by H2O2 and NaBH4.
  • Comprehensive photophysical properties of both emissive states were determined, confirming recoverability.

Conclusions:

  • The study presents a novel DNA-AgNC system with reversible red-to-green emission switching.
  • The findings highlight the potential of this system for creating advanced DNA-AgNC-based ratiometric sensors.
  • Understanding the conversion mechanism paves the way for new sensor designs utilizing switchable nanomaterials.