Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy

Noemi Molina1,2, Marvin Cnudde3, Juan A Guadix4,2

  • 1Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Málaga-IBIMA, Campus de Teatinos s/n, 29071 Málaga, Spain.

ACS Omega
|August 29, 2019
PubMed

Related Concept Videos

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization05:54

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

This paper describes how polarization-sensitive two-photon microscopy could be applied to characterize the local organization within label-free amyloid superstructures-spherulites. It also describes how to prepare and measure the sample, assemble the required setup, and analyze the data to obtain information about the local organization of amyloid...
1.7K
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy14:11

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved...
16.3K
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

We present principles of oxygen measurements by phosphorescence quenching and review design of porphyrin-based dendritic nanosensors for oxygen imaging in biological...
14.3K
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
586
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence08:58

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in...
15.2K
Two-Photon Microscopy for Imaging Neuronal Morphology in a Mouse Pup02:52

Two-Photon Microscopy for Imaging Neuronal Morphology in a Mouse Pup

Source: Mizuno, H., et al. In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice. J. Vis. Exp. (2018)This video showcases two-photon microscopy being used to image neurons in the brain of an anesthetized transgenic mouse pup. The two-photon laser allows for deeper imaging with high spatial resolution and minimal light scattering, enabling the detailed capture of neuronal morphology, including dendritic...
529