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Silicon Metal-oxide-semiconductor Quantum Dots for Single-electron Pumping
Published on: June 3, 2015
Coordination promiscuity guarantees metal substrate selection in transmembrane primary-active Zn2+ pumps
Marc J Gallenito1, Gordon W Irvine, Limei Zhang
1Department of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080, USA. Gabriele.meloni@utdallas.edu.
Abstract:
Metal selectivity in P1B-type ATPase pumps appears to be determined by amino acid motifs on their transmembrane helices. We reveal the principles governing substrate promiscuity towards first-, second- and third-row transition metals in a transmembrane Zn2+/Cd2+/Hg2+/Pb2+ P-type ATPase (ZntA), by dissecting its coordination chemistry. Atomic resolution characterization in detergent micelles and lipid bilayers reveals a "plastic" transmembrane metal-binding site that selects substrates by unique and diverse, yet defined, coordination geometries and ligand-metal distances.
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